Document Detail


Neuronal characteristics in embryonic renal stroma.
MedLine Citation:
PMID:  8074998     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The metanephric mesenchyme is considered a homogeneous population of predetermined, but pluripotent cells with a nephrogenic bias. By an inductive stimulus, the mesenchyme is programmed to differentiate into the various epithelial phenotypes of the secretory nephron. A fraction of the mesenchymal cells, however, remains in the interstitium between the nephrons and differentiates into spindle-shaped, clear-cytoplasmic renal stroma. We have analyzed the molecular nature of these cells in order to discover the specific cell types that could be involved in the morphogenetic processes during kidney differentiation. In situ hybridization reveals neurofilament light protein mRNA, and immunohistology shows neurofilament light and medium proteins in the stromal cells around kidney tubules. By immunohistochemistry these peritubular stromal cells can be distinguished from the neuronal cells of the renal microganglion: the peritubular stromal cells are neurofilament-positive but L1 neural cell adhesion protein-negative, whereas the neuronal cells with axonal extension are both neurofilament-positive and L1 neural cell adhesion protein-positive. Proliferation index of the stromal cells was low as compared to tubular cells, as shown by bromodeoxyuridine incorporation.
Authors:
K Sainio; D Nonclercq; M Saarma; J Palgi; L Saxén; H Sariola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  38     ISSN:  0214-6282     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-10-06     Completed Date:  1994-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  77-84     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion Molecules, Neuronal / analysis,  biosynthesis
Cell Differentiation
Epithelial Cells
Epithelium / physiology
Female
In Situ Hybridization
Kidney / embryology*,  innervation*
Male
Mice
Mice, Inbred CBA
Mice, Inbred Strains
Morphogenesis
Neurofilament Proteins / biosynthesis
Neurons / cytology*,  physiology
Phenotype
RNA, Messenger / analysis,  biosynthesis
Rats
Rats, Sprague-Dawley
Stem Cells / cytology,  physiology
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules, Neuronal; 0/Neurofilament Proteins; 0/RNA, Messenger; 0/neurofilament protein L

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