Document Detail

Neuronal ERCC6 mRNA expression in rat brain induced by a transient focal cerebral ischemia.
MedLine Citation:
PMID:  10437118     Owner:  NLM     Status:  MEDLINE    
AIM: To study whether the excision repair cross-complementing group 6 (ERCC6) is involved in the neuronal pathophysiological process following cerebral ischemia-reperfusion injury. METHODS: A transient middle cerebral artery occlusion (MCAO) was used to induce cerebral ischemia-reperfusion injury in rat brain. Northern blot was used to check a specific signal for oligonucleotide probe. The expression of ERCC6 mRNA in the rat brain was observed by in situ hybridization. The specific cellular distribution of ERCC6 mRNA in the neuron or glia of the rat brain was analyzed by double staining combined with confocal laser scanning microscopic analysis. RESULT: The expression of ERCC6 mRNA in the penumbra area increased following ischemia and reperfusion with a time-dependent manner. ERCC6 was expressed on d 2, reached peak values on d 3, and kept high level even on d 14 of reperfusion following ischemia. Number of ERCC6 mRNA expressive cell in the penumbra area on d 1, d 2, d 3, d 7, d 14 of reperfusion following ischemia were (0 +/- 0), (253 +/- 56), (816 +/- 355), (341 +/- 185), (128 +/- 95) x 10(6) cells/m2, respectively. Confocal microscopic analysis showed that ERCC6 mRNA coexpressed with phosphopyruvate hydratase in the neurons and with glial fibrillary acidic protein (GFAP) in a few proliferation astrocyte glia. CONCLUSION: The expression of transcription-repair coupling factor ERCC6 mRNA in the neuron and glia was induced by ischemia-reperfusion injury.
X Ling; L M Zhang; Y L Huang; W L Bao; F Y Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zhongguo yao li xue bao = Acta pharmacologica Sinica     Volume:  20     ISSN:  0253-9756     ISO Abbreviation:  Zhongguo Yao Li Xue Bao     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-12-09     Completed Date:  1999-12-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100330     Medline TA:  Zhongguo Yao Li Xue Bao     Country:  CHINA    
Other Details:
Languages:  eng     Pagination:  15-20     Citation Subset:  IM    
Department of Neurobiology, Shanghai Medical University, Shanghai Research Center for Life Science, China.
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MeSH Terms
Brain / metabolism*
DNA Helicases / biosynthesis*,  genetics
DNA Repair*
Ischemic Attack, Transient / metabolism*
Neurons / metabolism
RNA, Messenger / genetics
Rats, Sprague-Dawley
Reperfusion Injury / metabolism*
Reg. No./Substance:
0/RNA, Messenger; EC 3.6.1.-/DNA Helicases

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