Document Detail

Neuromyelitis optica pathology in rats following intraperitoneal injection of NMO-IgG and intracerebral needle injury.
MedLine Citation:
PMID:  24758159     Owner:  NLM     Status:  Publisher    
INTRODUCTION: Animal models of neuromyelitis optica (NMO) are needed for drug testing and evaluation of NMO disease pathogenesis mechanisms.
RESULTS: We describe a novel passive-transfer model of NMO in which rats made seropositive for human anti-aquaporin-4 (AQP4) immunoglobulin G antibody (NMO-IgG) by intraperitoneal (IP) injections were subject to intracerebral needle injury. Following a single IP injection, NMO-IgG distributed rapidly to peripheral AQP4-expressing cells (kidney collecting duct, gastric glands, airways, skeletal muscle) and area postrema in brain, but not elsewhere in the central nervous system; however, no pathology was seen in brain, spinal cord, optic nerve or peripheral tissues. After testing various maneuvers to produce NMO-IgG-dependent pathology in brain, we found that transient puncture of brain parenchyma with a 28-gauge needle in NMO-IgG seropositive rats produced robust NMO pathology around the needle track, with loss of AQP4 and glial fibrillary acidic protein, granulocyte and macrophage infiltration, centrovascular deposition of activated complement, and blood-brain barrier disruption, with demyelination by 5 days. Pathology was not seen in rats receiving control (non-NMO) human IgG or in NMO-IgG-seropositive rats made complement-deficient by cobra venom factor. Interestingly, at 1 day a reversible, multifocal astrocytopathy was seen with loss of AQP4 and GFAP (but not myelin) in areas away from the needle track.
CONCLUSIONS: NMO-IgG-seropositivity alone is not sufficient to cause NMO pathology in rats, but a single intracerebral needle insertion, without pre-existing inflammation or infusion of pro-inflammatory factors, was sufficient to produce robust NMO pathology in seropositive rats.
Nithi Asavapanumas; Alan S Verkman
Related Documents :
24126919 - Desire for social contact, not empathy, may explain "rescue" behavior in rats.
24512959 - Spontaneous intraocular hemorrhage in rats during postnatal ocular development.
23325329 - Treatment with tianeptine induces antidepressive-like effects and alters the neurotroph...
9389339 - Quantification of furosemide from serum and tissues using high-performance liquid chrom...
23488879 - Increased amplitude of the circadian variations in locomotor activity, systolic arteria...
24561489 - Behavioral and neural effects of intra-striatal infusion of anti-streptococcal antibodi...
7926599 - Ontogeny of vasoactive intestinal peptide receptors in rat ventral prostate.
3356369 - Unconjugated bilirubin and the bile from light exposed gunn rats inhibit intestinal wat...
2356749 - The effect of post-natal malnutrition on pepsinogen secretion receptors in weanling rats.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-4-24
Journal Detail:
Title:  Acta neuropathologica communications     Volume:  2     ISSN:  2051-5960     ISO Abbreviation:  Acta Neuropathol Commun     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101610673     Medline TA:  Acta Neuropathol Commun     Country:  -    
Other Details:
Languages:  ENG     Pagination:  48     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Opening minds stigma scale for health care providers (OMS-HC): examination of psychometric propertie...
Next Document:  Respondents' recall of injury events: an investigation of recall bias in cross-sectional injury data...