Document Detail

Neuromuscular junction morphology, fiber-type proportions, and satellite-cell proliferation rates are altered in MyoD(-/-) mice.
MedLine Citation:
PMID:  20544915     Owner:  NLM     Status:  MEDLINE    
Gene compensation by members of the myogenic regulatory factor (MRF) family has been proposed to explain the apparent normal adult phenotype of MyoD(-/-) mice. Nerve and field stimulation were used to investigate contraction properties of muscle from MyoD(-/-) mice, and molecular approaches were used to investigate satellite-cell behavior. We demonstrate that MyoD deletion results in major alterations in the organization of the neuromuscular junction, which have a dramatic influence on the physiological contractile properties of skeletal muscle. Second, we show that the lineage progression of satellite cells (especially initial proliferation) in the absence of MyoD is abnormal and linked to perturbations in the nuclear localization of beta-catenin, a key readout of canonical Wnt signaling. These results show that MyoD has unique functions in both developing and adult skeletal muscle that are not carried out by other members of the MRF family.
Raymond Macharia; Anthony Otto; Petr Valasek; Ketan Patel
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Muscle & nerve     Volume:  42     ISSN:  1097-4598     ISO Abbreviation:  Muscle Nerve     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-01     Completed Date:  2010-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7803146     Medline TA:  Muscle Nerve     Country:  United States    
Other Details:
Languages:  eng     Pagination:  38-52     Citation Subset:  IM    
Department of Veterinary Basic Sciences, Royal Veterinary College, London, NW1 0TU, UK.
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MeSH Terms
Cell Lineage
Cell Proliferation
Cells, Cultured
Electric Stimulation
Glycolysis / physiology
Mice, Inbred BALB C
Mice, Knockout
Microscopy, Electron
Muscle Contraction / physiology
Muscle Fibers, Skeletal / classification,  ultrastructure*
MyoD Protein / genetics*
Neuromuscular Junction / ultrastructure*
Nuclear Localization Signals / physiology
Satellite Cells, Skeletal Muscle / ultrastructure*
Succinate Dehydrogenase / metabolism
beta Catenin / biosynthesis,  genetics
Grant Support
078649//Wellcome Trust
Reg. No./Substance:
0/MyoD Protein; 0/MyoD1 myogenic differentiation protein; 0/Nuclear Localization Signals; 0/beta Catenin; EC Dehydrogenase

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