Document Detail

Neuroleukin inhibition sensitises neuronal cells to caspase-dependent apoptosis.
MedLine Citation:
PMID:  12615053     Owner:  NLM     Status:  MEDLINE    
Neuroleukin (NLK) is a multifunctional protein, involved in neuronal growth, glucose metabolism, cell motility, and differentiation. Expressed in the brain, it supports the growth of embryonic spinal, skeletal motor, and sensory neurons. We have previously demonstrated that NLK is up-regulated in the brain during Huntington's disease (HD), a neurodegenerative disorder caused by the expansion of CAG trinucleotide repeats. In order to study the biological role of NLK, we have generated an inducible rat pheochromocytoma PC12 cell line in which the expression of NLK is selectively down-regulated by antisense strategy. We show here that the block of NLK commits PC12 cells to caspase-dependent apoptosis. This priming effect elicited by NLK inhibition is independent from the differentiation state of the neuronal cells. These results suggest a general protective role of NLK in the control of cell death in neuronal cells.
Alessandra Romagnoli; Serafina Oliverio; Cristina Evangelisti; Carlo Iannicola; Giuseppe Ippolito; Mauro Piacentini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  302     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-04     Completed Date:  2003-05-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  448-53     Citation Subset:  IM    
Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy.
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MeSH Terms
Amino Acid Chloromethyl Ketones / pharmacology
Blotting, Western
Caspases / metabolism*
Cell Death
DNA, Complementary / metabolism
Electrophoresis, Polyacrylamide Gel
Flow Cytometry
Genetic Vectors
Green Fluorescent Proteins
Luminescent Proteins / metabolism
Microscopy, Fluorescence
Neuroleukin / antagonists & inhibitors*,  physiology*
Neurons / metabolism*
Oligonucleotides, Antisense / pharmacology
PC12 Cells
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Amino Acid Chloromethyl Ketones; 0/DNA, Complementary; 0/Luminescent Proteins; 0/Neuroleukin; 0/Oligonucleotides, Antisense; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; 147336-22-9/Green Fluorescent Proteins; EC 3.4.22.-/Caspases

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