| Neuroinflammation of the nigrostriatal pathway during progressive 6-OHDA dopamine degeneration in rats monitored by immunohistochemistry and PET imaging. | |
| | |
MedLine Citation:
|
PMID: 11918659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We investigated the microglial response to progressive dopamine neuron degeneration using in vivo positron emission tomography (PET) imaging and postmortem analyses in a Parkinson's disease (PD) rat model induced by unilateral (right side) intrastriatal administration of 6-hydroxydopamine (6-OHDA). Degeneration of the dopamine system was monitored by PET imaging of presynaptic dopamine transporters using a specific ligand (11)C-CFT (2beta-carbomethoxy-3beta-(4-fluorophenyl) tropane). Binding of (11)C-CFT was markedly reduced in the striatum indicating dopaminergic degeneration. Parallel PET studies of (11)C-PK11195 (1-(2-chlorophenyl)-N-methyl-N-(1-methylpropyl)-3 isoquinoline carboxamide) (specific ligand for activated microglia) showed increased binding in the striatum and substantia nigra indicative of a microglial response. Postmortem immunohistochemical analyses were performed with antibodies against CR3 for microglia/macrophage activation. Using a qualitative postmortem index for microglial activation we found an initially focal, then widespread microglial response at striatal and nigral levels at 4 weeks postlesion. These data support the hypothesis that inflammation is a significant component of progressive dopaminergic degeneration that can be monitored by PET imaging. |
| | |
Authors:
|
F Cicchetti; A L Brownell; K Williams; Y I Chen; E Livni; O Isacson |
Related Documents
:
|
21752889 - Functional imaging of sdhx-related head and neck paragangliomas: comparison of 18f-fluo... 15231499 - Tagged mri and pet in severe cad: discrepancy between preoperative inotropic reserve an... 7839959 - Noninvasive assessment of myocardial perfusion and metabolism: feasibility of registeri... 20927019 - Impact of pet/ct on planning of radiotherapy in head and neck cancer. 1601609 - Positron emission tomography as a diagnostic tool. a reassessment based on literature r... 20610039 - Reproducibility of "intelligent" contouring of gross tumor volume in non-small-cell lun... 17654589 - Development of a compact mri system for trabecular bone microstructure measurements of ... 2335599 - Gadolinium enhanced mr imaging of vascular stents. 21301969 - Magnetic resonance imaging and ultrasonography in predicting infiltrating residual dise... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The European journal of neuroscience Volume: 15 ISSN: 0953-816X ISO Abbreviation: Eur. J. Neurosci. Publication Date: 2002 Mar |
Date Detail:
|
Created Date: 2002-03-28 Completed Date: 2002-06-12 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8918110 Medline TA: Eur J Neurosci Country: France |
Other Details:
|
Languages: eng Pagination: 991-8 Citation Subset: IM |
Affiliation:
|
Neuroregeneration Laboratory, MRC119, McLean Hospital/Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD* Antigens, CD147 Antigens, Neoplasm* Antigens, Surface* Antineoplastic Agents / pharmacology Avian Proteins* Blood Proteins* Carbon Radioisotopes / diagnostic use Dopamine / metabolism* Encephalitis / pathology, physiopathology, radionuclide imaging* Gliosis / pathology, physiopathology, radionuclide imaging Isoquinolines / pharmacology Membrane Glycoproteins / metabolism Microglia / metabolism*, pathology Neostriatum / pathology, physiopathology, radionuclide imaging* Neural Pathways / pathology, physiopathology, radionuclide imaging* Neurons / metabolism*, pathology Oxidopamine / pharmacology Parkinson Disease / pathology, physiopathology, radionuclide imaging Rats Substantia Nigra / pathology, physiopathology, radionuclide imaging* Sympatholytics / pharmacology Tomography, Emission-Computed Tyrosine 3-Monooxygenase / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
NS 37654/NS/NINDS NIH HHS; NS 41263-02/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD; 0/Antigens, Neoplasm; 0/Antigens, Surface; 0/Antineoplastic Agents; 0/Avian Proteins; 0/Blood Proteins; 0/Bsg protein, Gallus gallus; 0/Bsg protein, rat; 0/Carbon Radioisotopes; 0/Isoquinolines; 0/Membrane Glycoproteins; 0/Sympatholytics; 1199-18-4/Oxidopamine; 136894-56-9/Antigens, CD147; 85340-56-3/PK 11195; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Synaptic depression improves coincidence detection in the nucleus laminaris in brainstem slices of t...
Next Document: Transistor array with an organotypic brain slice: field potential records and synaptic currents.