Document Detail


Neurogenesis impairment and increased cell death reduce total neuron number in the hippocampal region of fetuses with Down syndrome.
MedLine Citation:
PMID:  18093248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously obtained evidence for reduced cell proliferation in the dentate gyrus (DG) of fetuses with Down syndrome (DS), suggesting that the hippocampal hypoplasia seen in adulthood may be caused by defective early neuron production. The goal of this study was to establish whether DS fetuses (17-21 weeks of gestation) exhibit reduction in total cell number in the DG, hippocampus and parahippocampal gyrus (PHG). Volumes of the cellular layers and cell number were estimated with Cavalieri's principle and the optical fractionator method, respectively. We found that in DS fetuses all investigated structures had a reduced volume and cell number. Analysis of cell phenotype showed that DS fetuses had a higher percentage of cells with astrocytic phenotype but a smaller percentage of cells with neuronal phenotype. Immunohistochemistry for Ki-67, a marker of cycling cells, showed that DS fetuses had less proliferating cells in the germinal zones of the hippocampus and PHG. We additionally found that in the hippocampal region of DS fetuses there was a higher incidence of apoptotic cell death. Results show reduced neuron number in the DS hippocampal region and suggest that this defect is caused by disruption of neurogenesis and apoptosis, two fundamental processes underlying brain building.
Authors:
Sandra Guidi; Paola Bonasoni; Claudio Ceccarelli; Donatella Santini; Fabio Gualtieri; Elisabetta Ciani; Renata Bartesaghi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-17
Journal Detail:
Title:  Brain pathology (Zurich, Switzerland)     Volume:  18     ISSN:  1015-6305     ISO Abbreviation:  Brain Pathol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-26     Completed Date:  2008-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9216781     Medline TA:  Brain Pathol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  180-97     Citation Subset:  IM    
Affiliation:
Dipartimento di Fisiologia Umana e Generale, Università di Bologna, Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Body Weight
Case-Control Studies
Caspase 3 / metabolism
Cell Count
Cell Death
Cell Differentiation
Cell Proliferation*
Down Syndrome / pathology*
Female
Fetus / pathology*
Glial Fibrillary Acidic Protein / metabolism
Hippocampus / pathology*
Humans
Infant
Ki-67 Antigen / metabolism
Male
Neurons / metabolism,  pathology*
Phosphopyruvate Hydratase / metabolism
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Ki-67 Antigen; EC 3.4.22.-/Caspase 3; EC 4.2.1.11/Phosphopyruvate Hydratase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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