Document Detail

Neuroendocrine profile in a rat model of psychosocial stress: relation to oxidative stress.
MedLine Citation:
PMID:  23320850     Owner:  NLM     Status:  MEDLINE    
AIMS: Psychosocial stress alters the hypothalamic-pituitary-adrenal axis (HPA-axis). Increasing evidence shows a link between these alterations and oxidant elevation. Oxidative stress is implicated in the stress response and in the pathogenesis of neurologic and psychiatric diseases. NADPH oxidases (NOXs) are a major source of reactive oxygen species (ROS) in the central nervous system. Here, we investigated the contributory role of NOX2-derived ROS to the development of neuroendocrine alterations in a rat model of chronic psychosocial stress, the social isolation.
RESULTS: Significant elevations in the hypothalamic levels of corticotropin-releasing factor and plasmatic adrenocorticotropic hormone were observed from 4 weeks of social isolation. Increased levels of peripheral markers of the HPA-axis (plasmatic and salivary corticosterone) were observed at a later time point of social isolation (7 weeks). Alteration in the exploratory activity of isolated rats followed the same time course. Increased expression of markers of oxidative stress (8-hydroxy-2-deoxyguanosine [8OhdG] and nitrotyrosine) and NOX2 mRNA was early detectable in the hypothalamus of isolated rats (after 2 weeks), but later (after 7 weeks) in the adrenal gland. A 3-week treatment with the antioxidant/NOX inhibitor apocynin stopped the progression of isolation-induced alterations of the HPA-axis. Rats with a loss-of-function mutation in the NOX2 subunit p47(phox) were totally protected from the alterations of the neuroendocrine profile, behavior, and increased NOX2 mRNA expression induced by social isolation.
INNOVATION: We demonstrate that psychosocial stress induces early elevation of NOX2-derived oxidative stress in the hypothalamus and consequent alterations of the HPA-axis, leading ultimately to an altered behavior.
CONCLUSION: Pharmacological targeting of NOX2 might be of crucial importance for the treatment of psychosocial stress-induced psychosis.
Marilena Colaianna; Stefania Schiavone; Margherita Zotti; Paolo Tucci; Maria Grazia Morgese; Liselotte Bäckdahl; Rikard Holmdahl; Karl-Heinz Krause; Vincenzo Cuomo; Luigia Trabace
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Antioxidants & redox signaling     Volume:  18     ISSN:  1557-7716     ISO Abbreviation:  Antioxid. Redox Signal.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2013-08-26     Revised Date:  2014-04-22    
Medline Journal Info:
Nlm Unique ID:  100888899     Medline TA:  Antioxid Redox Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1385-99     Citation Subset:  IM    
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MeSH Terms
Acetophenones / pharmacology
Adrenal Glands / metabolism
Adrenocorticotropic Hormone / blood*
Antioxidants / pharmacology
Biological Markers / blood
Corticosterone / blood*
Deoxyguanosine / analogs & derivatives,  metabolism
Hypothalamo-Hypophyseal System / metabolism
Hypothalamus / metabolism
NADPH Oxidase / genetics,  metabolism
Neurosecretory Systems / metabolism
Oxidative Stress*
Pituitary-Adrenal System / metabolism
Psychosocial Deprivation
Psychotic Disorders / metabolism
Rats, Wistar
Restraint, Physical
Saliva / metabolism
Social Isolation
Stress, Psychological / blood*
Tyrosine / analogs & derivatives,  metabolism
Reg. No./Substance:
0/Acetophenones; 0/Antioxidants; 0/Biological Markers; 3604-79-3/3-nitrotyrosine; 42HK56048U/Tyrosine; 88847-89-6/8-oxo-7-hydrodeoxyguanosine; 9002-60-2/Adrenocorticotropic Hormone; B6J7B9UDTR/acetovanillone; EC Oxidase; EC cytosolic factor 1; G9481N71RO/Deoxyguanosine; W980KJ009P/Corticosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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