Document Detail

Neuroendocrine differentiation in bronchial carcinomas of classic squamous-cell type: an immunohistochemical study of 29 cases applying the tyramide signal amplification technique.
MedLine Citation:
PMID:  11277422     Owner:  NLM     Status:  MEDLINE    
With regard to the cellular origin of bronchial squamous-cell carcinomas, there are some clinicopathologic and experimental data indicating a link between neuroendocrine (NE) bronchial tumors and the traditionally non-NE squamous-cell carcinomas. Against this background, 29 consecutively resected bronchial squamous-cell carcinomas were examined immunohistochemically (IHC) by means of the specific NE cell marker chromogranin A (CgA), using not only conventional IHC methods, but also the technique with increased sensitivity, offered by the tyramide signal amplification (TSA) procedure. Whereas none of the 29 tumors displayed CgA immunoreactive (IR) cells using the conventional IHC procedure, 10 were found to display a fine granular CgA IR in the neoplastic parenchymal cells using the TSA technique. This incidence is higher than previously reported. However, the CgA IR cells never formed any majority cell population of the neoplastic parenchyma; when present, most of them occurred as micronodules or larger confluent areas in the peripheral most undifferentiated parts of the carcinomatous sheets. Single CgA IR cells were detected only rarely in the spinocellular or keratinized areas. It can be speculated that the observations conform with the recently proposed hypothesis that there is a reservoir of NE progenitor cells in the bronchial mucosa capable of proliferation.
A Fresvig; G Qvigstad; T B Halvorsen; S Falkmer; H L Waldum
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry     Volume:  9     ISSN:  1541-2016     ISO Abbreviation:  Appl. Immunohistochem. Mol. Morphol.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-29     Completed Date:  2001-04-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100888796     Medline TA:  Appl Immunohistochem Mol Morphol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9-13     Citation Subset:  IM    
Department of Intra-Abdominal Diseases, Trondheim University Hospital, Faculty of Medicine, Norwegian University of Science and Technology.
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MeSH Terms
Biotin / analogs & derivatives
Bronchial Neoplasms / metabolism*,  pathology*
Carcinoma, Small Cell / metabolism,  pathology
Carcinoma, Squamous Cell / metabolism*,  pathology*
Cell Differentiation
Chromogranin A
Chromogranins / metabolism
Immunohistochemistry / methods
Lung Neoplasms / metabolism,  pathology
Neurosecretory Systems / metabolism
Tumor Markers, Biological / metabolism
Tyramine / analogs & derivatives
Reg. No./Substance:
0/Chromogranin A; 0/Chromogranins; 0/Tumor Markers, Biological; 0/biotinyltyramide; 51-67-2/Tyramine; 58-85-5/Biotin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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