| Neurochemically distinct myenteric neurone populations containing calbindin have specific distribution patterns around the circumference of the gastric corpus. | |
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MedLine Citation:
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PMID: 11320647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We recently described calbindin immunoreactivity in the myenteric plexus of the guinea-pig stomach. To study the neurochemical coding of calbindin D28 k (CALB)-containing myenteric neurones, the presence of calretinin (CALRET), choline acetyltransferase (ChAT), enkephalin (ENK), neuropeptide Y, serotonin (5-HT), somatostatin (SOM) and substance P(SP) was investigated immunohistochemically in colchicine-treated preparations. Nitric oxide synthase-containing neurones were detected by NADPH-diaphorase histochemistry. In addition, we investigated the neurone distribution patterns around the gastric corpus. Most CALB neurones were ChAT positive. ChAT/CALB neurones were either CALRET (ca 75%) or 5-HT positive and most contained in addition SP and/or ENK. All 5-HT neurones contained CALB. CALB labelled on average 2.3, 4.8 and 7.5 neurones per ganglion at the lesser curvature, in the central region and the greater curvature, respectively, which indicated a preferential localisation at the greater curvature. Compared to the total number of myenteric neurones, the proportion of CALB neurones increased significantly from the lesser curvature (6%) towards the greater curvature (18%). This shift, although observed for most ChAT/CALB-positive populations, was most prominent for the ChAT/CALB/CALRET/SP/ENK-encoded neurones. SOM-positive and ChAT-only encoded neurones were preferentially located at the lesser curvature. The remaining ten neurochemically defined populations did not exhibit an uneven distribution. The colocalisation of CALB with CALRET or 5-HT is specific for myenteric neurones in the stomach and represents one significant difference to the neurochemical code of CALB neurones in the guinea-pig intestine. The functional significance of the unevenness of neurone distribution along the circumference of the gastric corpus remains to be studied. |
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Authors:
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D Reiche; K Huber; S Hoppe; M Schemann |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell and tissue research Volume: 303 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-04-25 Completed Date: 2001-09-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
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Languages: eng Pagination: 319-28 Citation Subset: IM |
Affiliation:
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Department of Physiology, School of Veterinary Medicine, Bischofsholer Damm 15, 30173 Hannover, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Calcium-Binding Protein, Vitamin D-Dependent / metabolism* Choline O-Acetyltransferase / metabolism Enkephalins / metabolism Enteric Nervous System / chemistry*, cytology Female Guinea Pigs Immunohistochemistry / methods Male Myenteric Plexus / chemistry*, cytology, metabolism Neurochemistry / methods Neurons / chemistry Neuropeptide Y / metabolism Neurotransmitter Agents / metabolism* Serotonin / metabolism Somatostatin / metabolism Stomach / innervation* Substance P / metabolism Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Enkephalins; 0/Neuropeptide Y; 0/Neurotransmitter Agents; 0/calbindin; 0/calretinin; 33507-63-0/Substance P; 50-67-9/Serotonin; 51110-01-1/Somatostatin; EC 2.3.1.6/Choline O-Acetyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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