| Neurochemical profile of the mouse hypothalamus using in vivo 1H MRS at 14.1T. | |
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MedLine Citation:
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PMID: 20235335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hypothalamus plays an essential role in the central nervous system of mammals by among others regulating glucose homeostasis, food intake, temperature, and to some extent blood pressure. Assessments of hypothalamic metabolism using, e.g. (1)H MRS in mouse models can provide important insights into its function. To date, direct in vivo (1)H MRS measurements of hypothalamus have not been reported. Here, we report that in vivo single voxel measurements of mouse hypothalamus are feasible using (1)H MRS at 14.1T. Localized (1)H MR spectra from hypothalamus were obtained unilaterally (2-2.2 microL, VOI) and bilaterally (4-4.4 microL) with a quality comparable to that of hippocampus (3-3.5 microL). Using LCModel, a neurochemical profile consisting of 21 metabolites was quantified for both hypothalamus and hippocampus with most of the Cramér-Rao lower bounds within 20%. Relative to the hippocampus, the hypothalamus was characterized by high gamma-aminobutryric acid and myo-inositol, and low taurine concentrations. When studying transgenic mice with no glucose transporter isoform 8 expressed, small metabolic changes were observed, yet glucose homeostasis was well maintained. We conclude that a specific neurochemical profile of mouse hypothalamus can be measured by (1)H MRS which will allow identifying and following metabolic alterations longitudinally in the hypothalamus of genetic modified models. |
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Authors:
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Hongxia Lei; Carol Poitry-Yamate; Frédéric Preitner; Bernard Thorens; Rolf Gruetter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: NMR in biomedicine Volume: 23 ISSN: 1099-1492 ISO Abbreviation: NMR Biomed Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8915233 Medline TA: NMR Biomed Country: England |
Other Details:
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Languages: eng Pagination: 578-83 Citation Subset: IM |
Affiliation:
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Laboratory of Functional and Metabolic Imaging (LIFMET), Institute of the Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. hongxia.lei@epfl.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Glucose Transport Proteins, Facilitative / genetics, metabolism Hypothalamus / anatomy & histology, chemistry*, metabolism Magnetic Resonance Spectroscopy / methods* Male Mice Mice, Inbred C57BL Mice, Knockout |
| Chemical | |
Reg. No./Substance:
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0/Glucose Transport Proteins, Facilitative; 0/Slc2a8 protein, mouse |
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