Document Detail


Neuroblastoma GOTO cells are hypersensitive to disruption of lipid rafts.
MedLine Citation:
PMID:  19706290     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GOTO cells, a neuroblastoma cell line retaining the ability to differentiate into neuronal or Schwann cells, were found to be rich in membrane rafts containing ganglioside GM2 and hypersensitive to lipid raft-disrupting methyl-beta-cyclodextrin (MbetaCD); the GM2-rich rafts and sensitivity to MbetaCD were markedly diminished upon their differentiation into Schwann cells. We first raised a monoclonal antibody that specifically binds to GOTO cells but not to differentiated Schwann cells and determined its target antigen as ganglioside GM2, which was shown to be highly concentrated in lipid rafts by its colocalization with flotillin, a marker protein of rafts. Disturbance of normal structure of the lipid raft by depleting its major constituent, cholesterol, with MbetaCD resulted in acute apoptotic cell death of GOTO cells, but little effects were seen on differentiated Schwann cells. Until this study, GM2-rich rafts are poorly characterized and MbetaCD hypersensitivity, which may have clinical implications, has not been reported.
Authors:
Ryosaku Tomioka; Natsumi Minami; Ai Kushida; Shiho Horibe; Ippei Izumi; Akira Kato; Keiko Fukushima; Hiroko Ideo; Katsuko Yamashita; Shigehisa Hirose; Yuji Saito
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-23
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  389     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-09-23     Completed Date:  2009-10-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  122-7     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Antigens, Neoplasm / immunology
Apoptosis*
Child
G(M2) Ganglioside / immunology
Humans
Membrane Microdomains / drug effects*,  ultrastructure*
Neuroblastoma / ultrastructure*
Schwann Cells / drug effects,  ultrastructure
beta-Cyclodextrins / pharmacology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/beta-Cyclodextrins; 0/methyl-beta-cyclodextrin; 19600-01-2/G(M2) Ganglioside

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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