Document Detail

Neuroanataomical distribution of the orphan GPR50 receptor in adult sheep and rodent brains.
MedLine Citation:
PMID:  22211985     Owner:  NLM     Status:  Publisher    
GPR50, formerly known as melatonin-related receptor, is one of the three subtypes of the melatonin receptor subfamily together with MT(1) and MT(2) receptors. In contrast to these two high affinity receptor subtypes and despite its high identity with the melatonin receptor family, GPR50 does not bind melatonin or any other known ligand. Specific and reliable immunological tools are therefore needed to be able to elucidate the physiological functions of this orphan receptor that are still largely unknown. We have generated and validated a new specific GPR50 antibody against the ovine GPR50 and used it to analyse the neuroanatomical distribution of the GPR50 in sheep, rat and mouse whole brain. We demonstrated that GPR50-positive cells are widely distributed in various regions including the hypothalamus and the pars tuberalis of the pituitary in all the three species studied. GPR50 expressing cells are abundant in the dorsomedial nucleus of the hypothalamus, the periventricular nucleus and the median eminence. In rodents, immunohistochemical studies revealed a broader distribution pattern for the GPR50 protein. GPR50 immunoreactivity is found in the medial preoptic area, the lateral spetum, lateral hypothalamic area, bed nucleus of the stria terminalis, in the vascular organ of the laminae terminalis and several regions of the amygdala, including medial nuclei of amygdala. Additionally, in the rat brain, GPR50 protein was localized in the CA1 pyramidal cell layer of the dorsal hippocampus. In mice, moderate to high numbers of GPR50-positive cells are also found in the subfornical organ. Altogether, these results provide an enlarged distribution of GPR50 protein, give further insight into the organization of the melatoninergic system and may lay the framework for future studies on the role of the GPR50 in the brain.
M Batailler; A Mullier; A Sidibe; P Delagrange; V Prévot; R Jockers; M Migaud
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-23
Journal Detail:
Title:  Journal of neuroendocrinology     Volume:  -     ISSN:  1365-2826     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8913461     Medline TA:  J Neuroendocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Journal of Neuroendocrinology © 2011 Blackwell Publishing.
INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France CNRS, UMR6175 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France Université François Rabelais de Tours, F-37041 Tours, France IFCE, F-37380 Nouzilly, France Inserm, Centre de Recherche Jean-Pierre Aubert, UMR837, Lille, France UDSL, Institut de Médecine Préventive et de Recherche Thérapeutique, Lille, France Univ. Lille Nord de France, Lille, France Inserm, U1016, Institut Cochin, Paris, France CNRS UMR 8104, Paris, France Univ. Paris Descartes, Sorbonne Paris Cite, Paris, France IDRS, Suresnes, France.
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