| Neurites outgrowth and amino acids levels in goldfish retina under hypo-osmotic or hyper-osmotic conditions. | |
| | |
MedLine Citation:
|
PMID: 22001617 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Amino acids are known to play relevant roles as osmolytes in various tissues, including the retina. Taurine is one of these active molecules. In addition, taurine stimulates outgrowth from the goldfish retina by mechanisms that include extracellular matrix, calcium fluxes and protein phosphorylation. The present report aims to explore the effect of medium osmolarity on goldfish retinal outgrowth and the possible modifications produced by changing eye osmolarity on amino acid levels in the retina. Goldfish retinal explants were obtained 10 days after crush of the optic nerve and cultured under iso-, hypo- or hyper-osmotic conditions. Hypo-osmotic medium was prepared by diluting the solutions 10% twice, preserving fetal calf serum concentration. Hyper-osmotic medium was done by adding 50 or 100mM urea or mannitol. Evaluation of length and density of neurites was performed 5 days after plating. Outgrowth was reduced in hypo- and in hyper-osmotic conditions. Taurine, 4mM, increased length and density of neurites in iso-osmotic, and produced stimulatory effects under both hyper-osmotic conditions. The in vivo modification of osmolarity by intraocular injection of water or 100mM urea modified levels of free amino acids in the retina. Taurine and aspartate retinal levels increased in a time-dependent manner after hypo- and hyper-osmotic solution injections. Serine, threonine, arginine, γ-aminobutyric acid, alanine and tyrosine were elevated in hyper-osmotic conditions. Outgrowth in vitro, after in vivo osmolarity changes, was higher in the absence of taurine, but did not increase in the presence of the amino acid. The fact that certain outgrowth took place in these conditions support that the impairment was not due to tissue damage. Rather, the effects might be related to the cascade of kinase events described during osmolarity variations. The time course under these conditions produced adjustments in ganglion cells probably related to taurine transporter, and phosphorylation of specific proteins. |
| | |
Authors:
|
Lisbeth Cubillán; Francisco Obregón; Lucimey Lima |
Related Documents
:
|
10607767 - Intradialytic removal of protein-bound uraemic toxins: role of solute characteristics a... 10365807 - Hepatic amino- to urea-n clearance and forearm amino-n exchange during hypoglycemic and... 3112427 - Studies on the toxicity and efficacy of a new amino acid solution in pediatric parenter... 2918517 - Studies on prodrugs. 10. possible mechanism of n-dealkylation of n-masked norfloxacins ... 9783127 - Taurine entry into perilymph of the guinea pig. 10234607 - Dietary management of long-chain 3-hydroxyacyl-coa dehydrogenase deficiency (lchadd). a... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-6 |
Journal Detail:
|
Title: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience Volume: - ISSN: 1873-474X ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8401784 Medline TA: Int J Dev Neurosci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 ISDN. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
|
Laboratorio de Neuroquímica, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones (IVIC), Apdo. 21827, Caracas 1020-A, Venezuela. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of pattern redundancy and hierarchical grouping on global-local visual processing in monkeys...
Next Document: Review of continent urinary diversion in contemporary urology.