Document Detail

Neurite differentiation is modulated in neuroblastoma cells engineered for altered acetylcholinesterase expression.
MedLine Citation:
PMID:  9326267     Owner:  NLM     Status:  MEDLINE    
Previous observations from several groups suggest that acetylcholinesterase (AChE) may have a role in neural morphogenesis, but not solely by virtue of its ability to hydrolyze acetylcholine. We tested the possibility that AChE influences neurite outgrowth in nonenzymatic ways. With this aim, antisense oligonucleotides were used to decrease AChE levels transiently, and N1E.115 cell lines were engineered for permanently altered AChE protein expression. Cells stably transfected with a sense AChE cDNA construct increased their AChE expression 2.5-fold over the wild type and displayed significantly increased neurite outgrowth. Levels of the differentiation marker, tau, also rose. In contrast, AChE expression in cell lines containing an antisense construct was half of that observed in the wild type. Significant reductions in neurite outgrowth and tau protein accompanied this effect. Overall, these measures correlated statistically with the AChE level (p < 0.01). Furthermore, treatment of AChE-overexpressing cells with a polyclonal antibody against AChE decreased neurite outgrowth by 43%. We conclude that AChE may have a novel, noncholinergic role in neuronal differentiation.
C Koenigsberger; S Chiappa; S Brimijoin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  69     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-10-27     Completed Date:  1997-10-27     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1389-97     Citation Subset:  IM    
Department of Pharmacology, Mayo Clinic, Rochester, Minnesota 55905, U.S.A.
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MeSH Terms
Acetylcholinesterase / genetics*,  metabolism*
Cholinesterase Inhibitors / pharmacology
Genetic Engineering*
Isoenzymes / metabolism
Neurites / enzymology*,  physiology*
Neuroblastoma / enzymology,  pathology*
Oligonucleotides, Antisense / pharmacology
Tissue Distribution
Tumor Cells, Cultured
tau Proteins / metabolism
Reg. No./Substance:
0/Cholinesterase Inhibitors; 0/Isoenzymes; 0/Oligonucleotides, Antisense; 0/tau Proteins; EC

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