Document Detail


Neurite outgrowth from PC12 cells is enhanced by an inhibitor of mechanical channels.
MedLine Citation:
PMID:  20600595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GsMTx4, a peptide inhibitor for mechanosensitive ion channels (MSCs), promoted neurite outgrowth from PC12 cells in the presence of NGF in a dose-dependent manner between 5 and 100 microM peptide. Enhanced neurite growth required >12 h of peptide exposure in cells grown with NGF. Adsorption of GsMTx4 to serum proteins in the media lowered the free peptide concentration of 100 microM to a free concentration of 5 microM, a concentration shown to completely inhibit MSCs in the patch clamp assay. Outside-out patches from PC12 cells grown in NGF had mechanically activated cation channels that were reversibly inhibited by GsMTx4. These results are similar to those observed by Gomez and co-workers in Xenopus spinal cord. The inhibition of mechanosensitive channels by GsMTx4 may be a useful approach to accelerate regeneration of neurons in neurodegenerative diseases and spinal cord injury.
Authors:
Philip A Gottlieb; Tara Barone; Frederick Sachs; Robert Plunkett
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Publication Detail:
Type:  Journal Article     Date:  2010-06-26
Journal Detail:
Title:  Neuroscience letters     Volume:  481     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-03     Completed Date:  2010-11-22     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  115-9     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Chromatography, High Pressure Liquid / methods
Dose-Response Relationship, Drug
Drug Synergism
Ion Channels / antagonists & inhibitors*
Membrane Potentials / drug effects,  physiology
Nerve Growth Factor / pharmacology
Neurites / drug effects*
PC12 Cells / cytology,  drug effects
Patch-Clamp Techniques / methods
Peptides / pharmacology*
Physical Stimulation / methods
Rats
Spider Venoms / pharmacology*
Time Factors
Grant Support
ID/Acronym/Agency:
R01 HL054887/HL/NHLBI NIH HHS; R01 HL054887-15/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Ion Channels; 0/MTx4 protein, Grammostola spatulata; 0/Peptides; 0/Spider Venoms; 9061-61-4/Nerve Growth Factor
Comments/Corrections

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