Document Detail


Neural tube closure in Xenopus laevis involves medial migration, directed protrusive activity, cell intercalation and convergent extension.
MedLine Citation:
PMID:  10498689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have characterized the cell movements and prospective cell identities as neural folds fuse during neural tube formation in Xenopus laevis. A newly developed whole-mount, two-color fluorescent RNA in situ hybridization method, visualized with confocal microscopy, shows that the dorsal neural tube gene xpax3 and the neural-crest-specific gene xslug are expressed far lateral to the medial site of neural fold fusion and that expression moves medially after fusion. To determine whether cell movements or dynamic changes in gene expression are responsible, we used low-light videomicroscopy followed by fluorescent in situ and confocal microscopy. These methods revealed that populations of prospective neural crest and dorsal neural tube cells near the lateral margin of the neural plate at the start of neurulation move to the dorsal midline using distinctive forms of motility. Before fold fusion, superficial neural cells apically contract, roll the neural plate into a trough and appear to pull the superficial epidermal cell sheet medially. After neural fold fusion, lateral deep neural cells move medially by radially intercalating between other neural cells using two types of motility. The neural crest cells migrate as individual cells toward the dorsal midline using medially directed monopolar protrusions. These movements combine the two lateral populations of neural crest into a single medial population that form the roof of the neural tube. The remaining cells of the dorsal neural tube extend protrusions both medially and laterally bringing about radial intercalation of deep and superficial cells to form a single-cell-layered, pseudostratified neural tube. While ours is the first description of medially directed cell migration during neural fold fusion and re-establishment of the neural tube, these complex cell behaviors may be involved during cavitation of the zebrafish neural keel and secondary neurulation in the posterior axis of chicken and mouse.
Authors:
L A Davidson; R E Keller
Related Documents :
20367289 - In vitro regulation of neural differentiation and axon growth by growth factors and bio...
6746879 - Fine structural aspects of the cranial neuroepithelium in early embryos of the rhesus m...
8777989 - High glucose concentration inhibits migration of rat cranial neural crest cells in vitro.
16197939 - The pattern of neural crest advance in the cecum and colon.
4018409 - Analysis of the origins and early fates of neural crest cells in caudal regions of avia...
2450659 - The cytoskeletal mechanics of brain morphogenesis. cell state splitters cause primary n...
22500829 - From sap-less t cells to helpless b cells and back: dynamic t-b cell interactions under...
16761719 - Polyethylene glycol-mediated cell fusion.
21197469 - Loss of function of e-cadherin in embryonic stem cells and the relevance to models of t...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  126     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-19     Completed Date:  1999-11-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4547-56     Citation Subset:  IM    
Affiliation:
Department of Biology, Gilmer Hall, University of Virginia, Charlottesville, VA 22903, USA. lance_davidson@virginia.edu.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning / genetics
Cell Movement / genetics
Central Nervous System / cytology,  embryology*
DNA-Binding Proteins / genetics
Gene Expression Regulation, Developmental
In Situ Hybridization, Fluorescence
Mice
Microscopy, Video
Neural Crest / cytology,  embryology
Paired Box Transcription Factors
Signal Transduction
Transcription Factors / genetics
Xenopus Proteins*
Xenopus laevis / embryology*,  genetics
Zebrafish Proteins*
Grant Support
ID/Acronym/Agency:
HD25595/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Paired Box Transcription Factors; 0/Pax3 protein, Xenopus; 0/Transcription Factors; 0/Xenopus Proteins; 0/Zebrafish Proteins; 0/pax3 protein, zebrafish; 0/snail family transcription factors; 138016-91-8/Pax3 protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Dissociation of MAP kinase activation and MPF activation in hormone-stimulated maturation of Xenopus...
Next Document:  Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and di...