| Neural transmembrane protease and endothelial Gs protein activation in cell contact-dependent signaling between neural stem/progenitor cells and brain endothelial cells. | |
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MedLine Citation:
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PMID: 22577149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vasculature is an important component of the neural stem cell niche in brain. It regulates neural stem/progenitor (NS/P) cell self-renewal, differentiation, and migration. In the neurogenic niches of adult brain, NS/P cells lie close to blood vessels, and proliferating NS/P cells frequently contact the vasculature. In the present study we showed that NS/P cells in co-culture with brain endothelial (bEND) cells activated endothelial G proteins and p38 mitogen-activated protein kinase (p38 MAPK) and stimulated cytokine/chemokine expression. These NS/P cell-induced endothelial responses took place during NS/P cell and bEND cell direct contact and were critically dependent on the expression of the type II transmembrane serine protease matriptase (MTP) by NS/P cells, because knocking down of MTP in NS/P cells impaired and re-expression of MTP restored their ability to induce endothelial cytokine/chemokine expression, p38 MAPK, or G protein activation. Cholera toxin blocked NS/P cell-induced endothelial responses, suggesting that the endothelial G protein activated by NS/P MTP is in the G(s) subfamily. The addition of p38 MAPK inhibitor impaired NS/P cell-induced endothelial cytokine/chemokine expression. The known G protein-coupled receptor substrate of MTP, protease-activated receptor 2, was not involved in this system. These results revealed a novel signaling pathway in neural stem cell vascular niches that is mediated by neural MTP and endothelial G(s) protein signaling at the cell-cell interface. This is the first report of direct cell-cell signaling between NS/P and bEND cells. |
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Authors:
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Hsiu-Hui Tung; Sheau-Ling Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-05-10 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 287 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-07-03 Completed Date: 2012-09-11 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 22497-508 Citation Subset: IM |
Affiliation:
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Institute of Cellular and Systems Medicine, National Health Research Institutes, Zhunan Town, Miaoli County 35053, Taiwan, Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / blood supply, cytology Cell Communication / physiology* Cell Differentiation / drug effects, physiology Cell Movement / physiology Cells, Cultured Chemokines / metabolism Coculture Techniques Culture Media, Conditioned / pharmacology Cytokines / metabolism Endothelial Cells / cytology*, metabolism* Ependyma / blood supply, cytology GTP-Binding Protein alpha Subunits, Gs / metabolism GTP-Binding Proteins / metabolism Green Fluorescent Proteins / genetics Interleukin-6 / pharmacology MAP Kinase Signaling System / physiology Male Mice Mice, Inbred Strains Mice, Mutant Strains Neural Stem Cells / cytology*, metabolism* Stem Cell Niche / physiology p38 Mitogen-Activated Protein Kinases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chemokines; 0/Culture Media, Conditioned; 0/Cytokines; 0/Interleukin-6; 147336-22-9/Green Fluorescent Proteins; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.5.1/GTP-Binding Protein alpha Subunits, Gs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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