Document Detail


Neural steroid hormone receptor gene expression in pregnant rats.
MedLine Citation:
PMID:  16253385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Estrogen and progesterone play important roles during pregnancy in stimulating the onset of maternal behavior at parturition. The status of receptor expression of these hormones during pregnancy in neural regions that regulate maternal behavior is unclear. The objective of the present study is to characterize changes in neural gene expression of the estrogen receptors alpha and beta (ERalpha and ERbeta) and the progesterone receptor (PR) during the latter part of pregnancy. Brains from primigravid Sprague-Dawley rats were collected on days 15 and 21 of pregnancy. Micropunches of the olfactory bulb (OB), medial preoptic area (MPOA), bed nucleus of the stria terminalis (BnST), hypothalamus (HYP), medial amygdala (MeA), and the temporal cortex (TCx) were analyzed by real-time RT-PCR (Taqmantrade mark) for levels of gene expression. No changes in either ERalpha or ERbeta mRNA levels were detected in any brain region between days 15 and 21 of pregnancy: however, the MPOA had higher levels of both ERalpha and ERbeta than other brain regions. Progesterone receptor mRNA levels, in contrast, declined significantly in the MPOA, HYP, and TCx, between days 15 and 21 of pregnancy (P < 0.05). In addition, the levels of PR mRNA were significantly higher in the HYP and TCx compared to both the OB and MeA. These data indicate that there is a downregulation of PR prepartum and suggest that this decrease may play a role in the disinhibition of maternal behavior at parturition.
Authors:
Phyllis E Mann; Jessica A Babb
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2005-10-25
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  142     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-21     Completed Date:  2006-02-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  39-46     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, Tufts University, Cummings School of Veterinary Medicine, N. Grafton, MA 01536, USA. Phyllis.Mann@Tufts.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / anatomy & histology,  metabolism*
Estrogen Receptor alpha / genetics,  metabolism
Estrogen Receptor beta / genetics,  metabolism
Female
Gene Expression / physiology*
Pregnancy
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Receptors, Progesterone / genetics,  metabolism
Receptors, Steroid / genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction / methods
Time Factors
Grant Support
ID/Acronym/Agency:
R01 HD39668/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/RNA, Messenger; 0/Receptors, Progesterone; 0/Receptors, Steroid

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