| Neural development is dependent on the function of specificity protein 2 in cell cycle progression. | |
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MedLine Citation:
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PMID: 23293287 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Faithful progression through the cell cycle is crucial to the maintenance and developmental potential of stem cells. Here, we demonstrate that neural stem cells (NSCs) and intermediate neural progenitor cells (NPCs) employ a zinc-finger transcription factor specificity protein 2 (Sp2) as a cell cycle regulator in two temporally and spatially distinct progenitor domains. Differential conditional deletion of Sp2 in early embryonic cerebral cortical progenitors, and perinatal olfactory bulb progenitors disrupted transitions through G1, G2 and M phases, whereas DNA synthesis appeared intact. Cell-autonomous function of Sp2 was identified by deletion of Sp2 using mosaic analysis with double markers, which clearly established that conditional Sp2-null NSCs and NPCs are M phase arrested in vivo. Importantly, conditional deletion of Sp2 led to a decline in the generation of NPCs and neurons in the developing and postnatal brains. Our findings implicate Sp2-dependent mechanisms as novel regulators of cell cycle progression, the absence of which disrupts neurogenesis in the embryonic and postnatal brain. |
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Authors:
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Huixuan Liang; Guanxi Xiao; Haifeng Yin; Simon Hippenmeyer; Jonathan M Horowitz; H Troy Ghashghaei |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 140 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-01-07 Completed Date: 2013-03-07 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 552-61 Citation Subset: IM |
Affiliation:
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Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / cytology, embryology, metabolism Cell Count Cell Cycle* Cell Proliferation Crosses, Genetic Embryo Implantation Embryo, Mammalian / cytology, metabolism Embryonic Stem Cells / cytology, metabolism Eye Proteins / genetics, metabolism Female Genetic Markers Homeodomain Proteins / genetics, metabolism Homologous Recombination Intermediate Filament Proteins / genetics, metabolism Mice Mice, Inbred C57BL Mice, Knockout Nerve Tissue Proteins / genetics, metabolism Neural Stem Cells / cytology, metabolism* Neurogenesis* Neurons / cytology, metabolism Paired Box Transcription Factors / genetics, metabolism Repressor Proteins / genetics, metabolism Sp2 Transcription Factor / genetics, metabolism* Stem Cell Niche Transplantation Chimera / embryology, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01NS062182/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Eye Proteins; 0/Genetic Markers; 0/Homeodomain Proteins; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/PAX6 protein; 0/Paired Box Transcription Factors; 0/Repressor Proteins; 0/nestin; 148710-93-4/Sp2 Transcription Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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