Document Detail

Neural development is dependent on the function of specificity protein 2 in cell cycle progression.
MedLine Citation:
PMID:  23293287     Owner:  NLM     Status:  MEDLINE    
Faithful progression through the cell cycle is crucial to the maintenance and developmental potential of stem cells. Here, we demonstrate that neural stem cells (NSCs) and intermediate neural progenitor cells (NPCs) employ a zinc-finger transcription factor specificity protein 2 (Sp2) as a cell cycle regulator in two temporally and spatially distinct progenitor domains. Differential conditional deletion of Sp2 in early embryonic cerebral cortical progenitors, and perinatal olfactory bulb progenitors disrupted transitions through G1, G2 and M phases, whereas DNA synthesis appeared intact. Cell-autonomous function of Sp2 was identified by deletion of Sp2 using mosaic analysis with double markers, which clearly established that conditional Sp2-null NSCs and NPCs are M phase arrested in vivo. Importantly, conditional deletion of Sp2 led to a decline in the generation of NPCs and neurons in the developing and postnatal brains. Our findings implicate Sp2-dependent mechanisms as novel regulators of cell cycle progression, the absence of which disrupts neurogenesis in the embryonic and postnatal brain.
Huixuan Liang; Guanxi Xiao; Haifeng Yin; Simon Hippenmeyer; Jonathan M Horowitz; H Troy Ghashghaei
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  140     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-07     Completed Date:  2013-03-07     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  552-61     Citation Subset:  IM    
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MeSH Terms
Brain / cytology,  embryology,  metabolism
Cell Count
Cell Cycle*
Cell Proliferation
Crosses, Genetic
Embryo Implantation
Embryo, Mammalian / cytology,  metabolism
Embryonic Stem Cells / cytology,  metabolism
Eye Proteins / genetics,  metabolism
Genetic Markers
Homeodomain Proteins / genetics,  metabolism
Homologous Recombination
Intermediate Filament Proteins / genetics,  metabolism
Mice, Inbred C57BL
Mice, Knockout
Nerve Tissue Proteins / genetics,  metabolism
Neural Stem Cells / cytology,  metabolism*
Neurons / cytology,  metabolism
Paired Box Transcription Factors / genetics,  metabolism
Repressor Proteins / genetics,  metabolism
Sp2 Transcription Factor / genetics,  metabolism*
Stem Cell Niche
Transplantation Chimera / embryology,  metabolism
Grant Support
Reg. No./Substance:
0/Eye Proteins; 0/Genetic Markers; 0/Homeodomain Proteins; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Nes protein, mouse; 0/Nestin; 0/PAX6 protein; 0/Paired Box Transcription Factors; 0/Repressor Proteins; 148710-93-4/Sp2 Transcription Factor

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