Document Detail


Neural changes in acute arthritis in monkeys. IV. Time-course of amino acid release into the lumbar dorsal horn.
MedLine Citation:
PMID:  1638274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extracellular levels of amino acids were measured during the development of experimental arthritis in anesthetized monkeys. Levels of glutamate, aspartate, glycine, serine, glutamine, taurine, cysteic acid and asparagine were each measured in consecutive 30 min samples before, during and for several hours after injection of kaolin and carrageenan into the articular capsule of one knee. Samples were obtained via a microdialysis probe placed in the lumbar dorsal horn ipsilateral to the injected knee and assayed using HPLC with fluorescence detection. Glutamate, aspartate, glycine and serine increased transiently following intra-articular injection of inflammatory agents. During this period glutamine levels decreased. A second phase of release then occurred which included more prolonged changes in amino acid levels that were sometimes of greater magnitude than those immediately following the injection. In animals which were later observed to have depletion of SP in the dorsal horn of the inflamed side, taurine levels increased starting after the Glu, Asp and Gly had plateaued at near baseline concentrations. Thus during the first stages of joint inflammation EAAs are released into the dorsal horn, followed by increased levels of IAAs, possibly representing activation of the descending endogenous analgesia system. This phase is followed by a semiacute response consisting in part of increased extracellular levels of SP and Tau. While SP is presumably part of an ascending nociceptive transmission system, Tau could be part of a second system aimed at reducing excessive neural activity including neural transmission resulting in intense maintained pain.
Authors:
L S Sorkin; K N Westlund; K A Sluka; P M Dougherty; W D Willis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research. Brain research reviews     Volume:  17     ISSN:  -     ISO Abbreviation:  Brain Res. Brain Res. Rev.     Publication Date:    1992 Jan-Apr
Date Detail:
Created Date:  1992-09-02     Completed Date:  1992-09-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8908638     Medline TA:  Brain Res Brain Res Rev     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  39-50     Citation Subset:  IM    
Affiliation:
Marine Biomedical Institute, University of Texas Medical Branch, Galveston 77550-2772.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Amino Acids / metabolism*
Animals
Arthritis / chemically induced,  metabolism*
Chromatography, High Pressure Liquid
Inflammation / metabolism
Injections
Lumbosacral Region
Macaca fascicularis
Movement / physiology
Neural Pathways / physiology
Spinal Cord / metabolism*
Taurine / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
NS01445/NS/NINDS NIH HHS; NS09743/NS/NINDS NIH HHS; NS11255/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 107-35-7/Taurine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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