Document Detail


Neural cell response to multiple novel sites on laminin-1.
MedLine Citation:
PMID:  10900077     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The basement membrane protein laminin-1 is a potent stimulator of neurite outgrowth for a variety of neuronal cell types. Previous studies have identified neurite outgrowth activity in several distinct regions of the laminin-1 molecule. In this study, 545 overlapping 12- to 14-mer synthetic peptides, corresponding to most of the amino acid sequence of the alpha1, beta1, and gamma1 chains of laminin-1, were screened for cell attachment and neurite outgrowth activity using primary cultures of mouse cerebellar granule neurons and two neuronal cell lines. We identified 48 peptides derived from novel regions of the laminin-1 molecule that were positive for neural cell adhesion activity. Only the cerebellar cells were found to have true neurite outgrowth activity with certain of the peptides, whereas some peptides induced short spike-like process with the cell lines. Although 23 of these peptides were active on all 3 cell types screened, 25 others showed cell-type specificity in their activity. These studies show that (1) there are multiple and distinct sites on laminin-1 for cell adhesion and neurite-like outgrowth and (2) that there are neural cell-type-specific active domains. The multiple active sites found explains, in part, the potent activity of laminin-1 on neurite outgrowth.
Authors:
S K Powell; J Rao; E Roque; M Nomizu; Y Kuratomi; Y Yamada; H K Kleinman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  61     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-08-29     Completed Date:  2000-08-29     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  302-12     Citation Subset:  IM    
Affiliation:
Craniofacial Developmental Biology and Regeneration Branch, National Institute for Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Adhesion
Cell Line
Laminin / chemistry,  metabolism*,  pharmacology
Neurites / physiology
Neurons / cytology*,  physiology,  ultrastructure
PC12 Cells
Peptide Fragments / chemistry,  pharmacology
Rats
Chemical
Reg. No./Substance:
0/Laminin; 0/Peptide Fragments; 0/laminin 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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