Document Detail

Neural cell protective compounds isolated from Phoenix hanceana var. formosana.
MedLine Citation:
PMID:  19628235     Owner:  NLM     Status:  MEDLINE    
A platform for screening drugs for their ability to protect neuronal cells against cytotoxicity was developed. Nerve growth factor (NGF) differentiates PC12 cells into nerves, and these differentiated PC12 cells enter apoptosis when challenged with 6-hydroxydopamine (6-OHDA). A screening spectrophotometer was used to assay cytotoxicity in these cells; pretreatment with test samples allowed identification of compounds that protected against this neuronal cytotoxicity. The 95% ethanol extract of Phoenix hanceana Naudin var. formosana Beccari. (PH) showed potential neuroprotective activity in these assays. The PH ethanol extract was further fractionated by sequential partitioning with n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and water. Subsequent rounds of assaying resulted in the isolation of ten constituents, and their structures were characterized by various spectroscopic techniques and identified by comparison with previous data as: isoorientin (1), isovitexin (2), veronicastroside (3), luteolin-7-O-beta-d-glucopyranoside (4), isoquercitrin (5), tricin-7-neohesperidoside (6), tricin-7-O-beta-d-gluco-pyranoside (7), (+)-catechin (8), (-)-epicatechin (9), and orientin 7-O-beta-d-glucopyranoside (10). Among these compounds, isovitexin (2), luteolin-7-O-beta-d-glucopyranoside (4) and (+)-catechin (8) showed significant neuroprotective activity in cell viability (WST-8 reduction), anti-apoptosis (Annexin V-FITC/propidium iodide double-labeled flow cytometry), and cellular ROS scavenging assays (besides isovitexin (2)), as well as a decreased caspase-8 activity in 6-OHDA-induced PC12 cells. Hence, isovitexin (2), luteolin-7-O-beta-d-glucopyranoside (4), and (+)-catechin (8) protected PC12 cells from 6-OHDA-induced apoptotic neurotoxicity.
Yi-Pei Lin; Tai-Yuan Chen; Hsiang-Wen Tseng; Mei-Hsien Lee; Shui-Tein Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-21
Journal Detail:
Title:  Phytochemistry     Volume:  70     ISSN:  1873-3700     ISO Abbreviation:  Phytochemistry     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-08-17     Completed Date:  2009-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1173-81     Citation Subset:  IM    
School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan.
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MeSH Terms
Angiosperms / chemistry*
Apigenin / chemistry,  isolation & purification,  pharmacology
Apoptosis / drug effects
Catechin / chemistry,  isolation & purification,  pharmacology
Glucosides / chemistry,  isolation & purification*,  pharmacology*
Luteolin / chemistry,  isolation & purification,  pharmacology
Molecular Structure
Neuroprotective Agents / chemistry,  isolation & purification*,  pharmacology*
Oxidopamine / pharmacology*
PC12 Cells
Plants, Medicinal / chemistry*
Quercetin / analogs & derivatives,  chemistry,  isolation & purification,  pharmacology
Reg. No./Substance:
0/Glucosides; 0/Neuroprotective Agents; 117-39-5/Quercetin; 1199-18-4/Oxidopamine; 154-23-4/Catechin; 21637-25-2/isoquercitrin; 38953-85-4/isovitexin; 4261-42-1/homoorientin; 491-70-3/Luteolin; 520-36-5/Apigenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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