Document Detail


Netrins guide migration of distinct glial cells in the Drosophila embryo.
MedLine Citation:
PMID:  20223758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Development of the nervous system and establishment of complex neuronal networks require the concerted activity of different signalling events and guidance cues, which include Netrins and their receptors. In Drosophila, two Netrins are expressed during embryogenesis by cells of the ventral midline and serve as attractant or repellent cues for navigating axons. We asked whether glial cells, which are also motile, are guided by similar cues to axons, and analysed the influence of Netrins and their receptors on glial cell migration during embryonic development. We show that in Netrin mutants, two distinct populations of glial cells are affected: longitudinal glia (LG) fail to migrate medially in the early stages of neurogenesis, whereas distinct embryonic peripheral glia (ePG) do not properly migrate laterally into the periphery. We further show that early Netrin-dependent guidance of LG requires expression of the receptor Frazzled (Fra) already in the precursor cell. At these early stages, Netrins are not yet expressed by cells of the ventral midline and we provide evidence for a novel Netrin source within the neurogenic region that includes neuroblasts. Later in development, most ePG transiently express uncoordinated 5 (unc5) during their migratory phase. In unc5 mutants, however, two of these cells in particular exhibit defective migration and stall in, or close to, the central nervous system. Both phenotypes are reversible in cell-specific rescue experiments, indicating that Netrin-mediated signalling via Fra (in LG) or Unc5 (in ePG) is a cell-autonomous effect.
Authors:
Christian M von Hilchen; Irina Hein; Gerhard M Technau; Benjamin Altenhein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-10
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  137     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-24     Completed Date:  2010-04-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  1251-62     Citation Subset:  IM    
Affiliation:
Institute of Genetics, University of Mainz, Saarstrasse 21, Mainz, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement
Cell Polarity
Cues
Drosophila / embryology*,  genetics,  physiology
Exons / genetics
Immunohistochemistry
Mutation
Nerve Growth Factors / physiology*
Neuroglia / cytology,  physiology*
Phenotype
Signal Transduction
Tumor Suppressor Proteins / physiology
Chemical
Reg. No./Substance:
0/Nerve Growth Factors; 0/Tumor Suppressor Proteins; 158651-98-0/netrin-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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