Document Detail


Netrin-guided accessory cell morphogenesis dictates the dendrite orientation and migration of a Drosophila sensory neuron.
MedLine Citation:
PMID:  20530550     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accessory cells, which include glia and other cell types that develop in close association with neurons, have been shown to play key roles in regulating neuron development. However, the underlying molecular and cellular mechanisms remain poorly understood. A particularly intimate association between accessory cells and neurons is found in insect chordotonal organs. We have found that the cap cell, one of two accessory cells of v'ch1, a chordotonal organ in the Drosophila embryo, strongly influences the development of its associated neuron. As it projects a long dorsally directed cellular extension, the cap cell reorients the dendrite of the v'ch1 neuron and tows its cell body dorsally. Cap cell morphogenesis is regulated by Netrin-A, which is produced by epidermal cells at the destination of the cap cell process. In Netrin-A mutant embryos, the cap cell forms an aberrant, ventrally directed process. As the cap cell maintains a close physical connection with the tip of the dendrite, the latter is dragged into an abnormal position and orientation, and the neuron fails to undergo its normal dorsal migration. Misexpression of Netrin-A in oenocytes, secretory cells that lie ventral to the cap cell, leads to aberrant cap cell morphogenesis, suggesting that Netrin-A acts as an instructive cue to direct the growth of the cap cell process. The netrin receptor Frazzled is required for normal cap cell morphogenesis, and mutant rescue experiments indicate that it acts in a cell-autonomous fashion.
Authors:
Eli M Mrkusich; Zalina B Osman; Karen E Bates; Julia M Marchingo; Molly Duman-Scheel; Paul M Whitington
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  137     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-09     Completed Date:  2010-06-22     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2227-35     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, University of Melbourne, VIC 3010, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement
Dendrites / metabolism*
Drosophila melanogaster / embryology*,  metabolism*
Nerve Growth Factors / metabolism*
Receptors, Cell Surface / metabolism
Sensory Receptor Cells / metabolism
Tumor Suppressor Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
R01AI081795-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Nerve Growth Factors; 0/Receptors, Cell Surface; 0/Tumor Suppressor Proteins; 0/netrin receptors; 158651-98-0/netrin-1
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Frizzled-5, a receptor for the synaptic organizer Wnt7a, regulates activity-mediated synaptogenesis.
Next Document:  Nuclear pre-mRNA 3'-end processing regulates synapse and axon development in C. elegans.