| Net uptake of gamma-aminobutyric acid by a high-affinity system of rat brain synaptosomes. | |
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MedLine Citation:
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PMID: 6940139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat brain synaptosomes isolated on discontinuous Ficoll gradient carry out rapid net uptake of gamma-aminobutyric acid through a high-affinity system (Km = 6.25 microM; Vmax = 1.2 nmol/min per mg of protein). The uptake of the labeled neurotransmitter is dependent on sodium concentration and is abolished by addition of 40 microM veratridine or 0.5 mM 2,4-diaminobutyric acid. Homoexchange in this preparation accounts for less than 10% of the measured uptake of gamma-amino[14C]butyric acid. It is concluded that the high-affinity transport exhibits properties characteristic of a system that is responsible for the rapid removal of gamma-aminobutyric acid from the synaptic cleft after neuronal transmission. |
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Authors:
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A Pastuszko; D F Wilson; M Erecińska |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 78 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1981 Feb |
Date Detail:
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Created Date: 1981-07-09 Completed Date: 1981-07-09 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1242-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminobutyric Acids
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pharmacology Animals Biological Transport / drug effects Brain / metabolism* Kinetics Male Rats Synaptosomes / drug effects, metabolism* Veratridine / pharmacology gamma-Aminobutyric Acid / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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NS-14505/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aminobutyric Acids; 305-62-4/2,4-diaminobutyric acid; 56-12-2/gamma-Aminobutyric Acid; 71-62-5/Veratridine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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