Document Detail

Nestin, a neuroectodermal stem cell marker molecule, is expressed in Leydig cells of the human testis and in some specific cell types from human testicular tumours.
MedLine Citation:
PMID:  15127288     Owner:  NLM     Status:  MEDLINE    
The intermediate filament protein nestin is predominantly expressed in some stem/progenitor cells and appears to be a useful molecular tool to characterise tumours originating from precursor cells of neuroectodermal and mesenchymal lineages. Leydig cells originate in the adult testis by differentiation from stem cells and express a variety of neural and neuroendocrine markers. The possible expression of the neural stem cell marker nestin in Leydig cells and testicular tumour cells was determined by analysing the patterns of nestin expression in normal and pathological human testes by Western blot and immunohistochemical methods. In normal testis, nestin was found in some vascular endothelial cells, a subset of peritubular spindle-shaped cells and some Leydig cells; spermatogenic and Sertoli cells were unstained. In normal Leydig cells, nestin was distributed in the perinuclear cytoplasm and accumulated in the crystalloids of Reinke with ageing. In non-tumour pathologies (cryptorchidism, impaired spermatogenesis), the seminiferous tubules were immunonegative, whereas hyperplastic Leydig cells showed cytoplasmic immunolabelling. In testicular malignancies, nestin was localised in the Sertoli cells of the seminiferous tubules affected with intratubular germ cell neoplasia, in the hyperplastic Leydig cells associated with these tumours and in some components (mesenchymal and neuroepithelial cells) of teratomas; spermatocytic and non-spermatocytic seminomas were unstained. Some vascular endothelial cells were immunolabelled in all tumour samples. Thus, nestin is expressed in a population of normal and hyperplastic Leydig cells and in Sertoli cells in the presence of intratubular germ-cell neoplasia. Nestin may be a good marker for identifying components of testicular teratomas.
Maria V T Lobo; Maria I Arenas; F Javier M Alonso; Gonzalo Gomez; Eulalia Bazán; Carlos L Paíno; Eldiberto Fernández; Benito Fraile; Ricardo Paniagua; Alfredo Moyano; Enrique Caso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-05-04
Journal Detail:
Title:  Cell and tissue research     Volume:  316     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-01     Completed Date:  2005-02-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  369-76     Citation Subset:  IM    
Departamento de Biología Celular y Genética, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
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MeSH Terms
Antigens, Differentiation / metabolism*
Ectoderm / metabolism
Endothelial Cells / metabolism,  pathology
Germ Cells / metabolism,  pathology
Intermediate Filament Proteins / metabolism*
Leydig Cells / metabolism*,  pathology
Middle Aged
Neoplasms, Germ Cell and Embryonal / metabolism,  pathology
Nerve Tissue Proteins / metabolism*
Seminiferous Tubules / metabolism,  pathology
Sertoli Cells / metabolism,  pathology
Stem Cells / cytology,  metabolism
Teratoma / metabolism*,  pathology
Testicular Neoplasms / metabolism*,  pathology
Tumor Markers, Biological / metabolism*
Reg. No./Substance:
0/Antigens, Differentiation; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Tumor Markers, Biological; 0/nestin

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