| Nested polymerase chain reaction in the diagnosis of cervical tuberculous lymphadenitis in Mexican children. | |
| | |
MedLine Citation:
|
PMID: 18926043 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
SETTING: Cervical tuberculous lymphadenitis (CTBL) diagnosis is a critical problem due to the difficulty in culturing Mycobacterium tuberculosis. OBJECTIVE: To evaluate a nested polymerase chain reaction (nPCR) for the diagnosis of CTBL. DESIGN: Thirty-eight children initially diagnosed on the basis of clinical and laboratory criteria as suffering from chronic cervical lymphadenitis were included in the study. Forty-one cervical lymph node specimens were analysed by bacterial staining, culture, cytology or histopathology. The IS6110 DNA sequence of M. tuberculosis complex was amplified by nPCR. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and efficiency were determined for the assay. RESULTS: The sensitivity of nPCR was 96%, the specificity 93%, PPV 96%, NPV 93% and efficiency 95%. Among 25 patients with CTBL, six presented a 'definite' diagnosis (24%) according to established criteria; 10 were classified as 'highly probable' cases (40%) and nine presented a 'possible' diagnosis (36%). The sensitivity of nPCR was higher than the sensitivity of staining (15%), culture (26%) and cytology or histopathology (62.5%) (95%CI P < 0.05, chi(2) P < 0.001). CONCLUSION: The nPCR used is a highly sensitive, specific and efficient method for the diagnosis of CTBL among children. |
| | |
Authors:
|
L Portillo-Gómez; M V Murillo-Neri; J Gaitan-Mesa; E G Sosa-Iglesias |
Related Documents
:
|
12195883 - Isolated cervical lymphadenopathy as unique manifestation of brucellosis. 17405603 - Conservative treatment of a cervical thoracic duct cyst: a case report. 4071103 - Meniscal tears on knee arthrography: patterns of arthrographic abnormalities. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Volume: 12 ISSN: 1027-3719 ISO Abbreviation: Int. J. Tuberc. Lung Dis. Publication Date: 2008 Nov |
Date Detail:
|
Created Date: 2008-10-17 Completed Date: 2009-02-04 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9706389 Medline TA: Int J Tuberc Lung Dis Country: France |
Other Details:
|
Languages: eng Pagination: 1313-9 Citation Subset: IM |
Affiliation:
|
Laboratorio de Microbiología y Parasitología, Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico. lpgegsi@prodigy.net.mx |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Child Child, Preschool Female Humans Infant Male Mexico Mycobacterium tuberculosis / isolation & purification* Polymerase Chain Reaction / methods* Predictive Value of Tests Prospective Studies Sensitivity and Specificity Tuberculosis, Lymph Node / diagnosis* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparison of MAS-PCR and GenoType MTBDR assay for the detection of rifampicin-resistant Mycobacteri...
Next Document: Value of chest radiography in predicting treatment response in children aged 3-59 months with severe...