| Nested cytochrome B polymerase chain reaction diagnostics detect sporozoites of hemosporidian parasites in peripheral blood of naturally infected birds. | |
| | |
MedLine Citation:
|
PMID: 19522549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Some discrepancies between microscopy and PCR-based methods have been recently recorded in the diagnosis of Leucocytozoon spp. infection in naturally infected birds. To clarify this issue, blood samples from 109 yellow-whiskered greenbuls Andropadus latirostris were investigated using both the microscopic examination of blood films and a nested mitochondrial cytochrome b PCR. The overall prevalence of Leucocytozoon spp. infection was 4% after the standard microscopic examination and 17% using the PCR diagnostics. Samples from 9 randomly chosen birds that were microscopy negative, but PCR positive, were then examined microscopically by screening 2 entire blood films from each individual bird. Sporozoites of Leucocytozoon spp. were observed in 4 birds, and 1 gametocyte of the parasite was seen in each of 2 birds. We conclude that sensitive PCR-based diagnostics are able to detect extremely light parasitemias of circulating sporozoites and gametocytes of hemosporidian parasites. Because of the PCR detection of sporozoites of unknown fate in the peripheral circulation, conclusions regarding the distribution of hemosporidian lineages in wildlife should be made with caution. To be accepted as the lineages of successfully developing species of hemosporidians, such PCR-based information should be supported with the detection of blood stages of the parasites. The present study emphasizes the crucial need for a synthesis of information provided by the tools of traditional parasitology and molecular biology, particularly in field studies of blood parasites. |
| | |
Authors:
|
Gediminas Valkiūnas; Tatjana A Iezhova; Claire Loiseau; Ravinder N M Sehgal |
Related Documents
:
|
12621669 - Sensitivity of polymerase chain reaction for detection of known aliquots of trypanosoma... 16899139 - The life-cycle of philornis downsi (diptera: muscidae) parasitizing darwin's finches an... 18779979 - An atypical microfilaria in blood samples from inhabitants of brazilian amazon. 10216109 - Impairment of plasmodium falciparum growth in thalassemic red blood cells: further evid... 839619 - Trypanosoma cervi kingston and morton, 1975 from mule deer, odocoileus hemionus, in wyo... 11473389 - Flow cytometric enumeration of micronucleated reticulocytes: high transferability among... 3998839 - Indomethacin-mediated improvement following middle cerebral artery occlusion in cats. e... 19867869 - The effect of heat on the transforming and binding power of blood. 10677769 - Chagas infection transmission control: situation of transfusional transmission in brazi... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: The Journal of parasitology Volume: 95 ISSN: 1937-2345 ISO Abbreviation: J. Parasitol. Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2010-10-08 Completed Date: 2010-11-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7803124 Medline TA: J Parasitol Country: United States |
Other Details:
|
Languages: eng Pagination: 1512-5 Citation Subset: IM |
Affiliation:
|
Institute of Ecology, Vilnius University, Akademijos 2, Vilnius 21, Lithuania. gedvalk@ekoi.lt |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/DQ847239; FJ839445; FJ839446; FJ839447; FJ839448; FJ839449; FJ839450; FJ839451 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Bird Diseases / diagnosis*, epidemiology, parasitology Cytochromes b / genetics DNA, Mitochondrial / chemistry DNA, Protozoan / blood, isolation & purification Ghana / epidemiology Haemosporida / genetics, isolation & purification* Molecular Sequence Data Parasitemia / diagnosis, epidemiology, parasitology, veterinary* Passeriformes / parasitology* Polymerase Chain Reaction / veterinary* Prevalence Protozoan Infections, Animal / diagnosis*, epidemiology, parasitology |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Mitochondrial; 0/DNA, Protozoan; 9035-37-4/Cytochromes b |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Metabolic profiles of serum from rats after subchronic exposure to chlorpyrifos and carbaryl.
Next Document: Effects of lipid-lowering pharmaceuticals bezafibrate and clofibric acid on lipid metabolism in fath...