| Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region. | |
| | |
MedLine Citation:
|
PMID: 20945000 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly sensitive and specific and it detects low-level infections in both humans and vectors. No cross-amplifications were observed with various other blood parasites and no false-positive results were obtained with the nested PCR. The method works efficiently with whole-blood, blood-spot and skin biopsy samples. Our method may thus be suitable for assessing the efficacy of filaria control programmes in Amazonia by recording parasite infections in both the human host and the vector. By specifically differentiating the major sympatric species of filaria, this technique could also enhance epidemiological research in the region. |
| | |
Authors:
|
Thuy-Huong Ta Tang; Rogelio López-Vélez; Marta Lanza; Anthony John Shelley; Jose Miguel Rubio; Sérgio Luiz Bessa Luz |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Memórias do Instituto Oswaldo Cruz Volume: 105 ISSN: 1678-8060 ISO Abbreviation: Mem. Inst. Oswaldo Cruz Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-10-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7502619 Medline TA: Mem Inst Oswaldo Cruz Country: Brazil |
Other Details:
|
Languages: eng Pagination: 823-8 Citation Subset: IM |
Affiliation:
|
Malaria y Protozoos Emergentes, Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo km 2, Majadahonda, Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Antigenic extracts of Leishmania braziliensis and Leishmania amazonensis associated with saponin par...
Next Document: Serologic evidence of the recent circulation of Saint Louis encephalitis virus and high prevalence o...