Document Detail

Nerve growth factor synthesis by intestinal epithelial cells.
MedLine Citation:
PMID:  7573456     Owner:  NLM     Status:  MEDLINE    
Nerve growth factor (NGF) exists in the gut of adult rats. The cells responsible for NGF synthesis in the gut remain unknown. IEC-6 and Caco-2 cells, established cell culture models of intestinal epithelial cells, were studied to determine whether intestinal epithelial cells, were studied to determine whether they synthesize and release NGF. Conditioned media from both IEC-6 and Caco-2 cells stimulated neurite outgrowth in both rat pheochromocytoma (PC-12) cells and sensory neurons derived from embryonic chick dorsal root ganglia (DRG). The addition of anti-NGF antibody blocked neurite outgrowth in PC-12 cells and partially blocked outgrowth in DRG cells. An NGF-enzyme-linked immunosorbant assay readily detected immunoreactive NGF in conditioned media from both cell lines, whereas cellular extracts from IEC-6, Caco-2, and isolated rat intestinal epithelial cells had low levels of immunoreactivity. Caco-2 monolayers primarily secreted NGF from the basolateral compartment, and interleukin-1 enhanced its secretion. IEC-6, Caco-2, and isolated rat intestinal epithelial cells expressed NGF mRNA as determined by reverse transcription polymerase chain reaction. These observations suggest that intestinal epithelial cells are capable of NGF synthesis.
G W Varilek; G A Neil; W P Bishop; J Lin; N J Pantazis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  269     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-11-06     Completed Date:  1995-11-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G445-52     Citation Subset:  IM    
Department of Internal Medicine, University of Kentucky College of Medicine, Lexington 40536-0084, USA.
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MeSH Terms
Caco-2 Cells
Cell Line
Cell Polarity
Cell Separation
Chick Embryo
Culture Media / pharmacology
Interleukin-1 / pharmacology
Intestinal Mucosa / cytology,  metabolism*
Nerve Growth Factors / genetics,  metabolism*
Neurites / drug effects,  physiology
PC12 Cells
RNA, Messenger / metabolism
Grant Support
Reg. No./Substance:
0/Culture Media; 0/Interleukin-1; 0/Nerve Growth Factors; 0/RNA, Messenger

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