Document Detail


Nerve growth factor induces proliferation of PC12 cells through Cdc42.
MedLine Citation:
PMID:  12824775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nerve growth factor (NGF) can change neurite outgrowth and cellular morphology of rat adrenal pheochromocytoma PC12 cells. In the present study, stable PC12 cells with constitutively active form (L61Cdc42), the dominant negative constructs (N17Cdc42), and wild type Cdc42 did not proliferate in low serum condition. However, on exposure to NGF, PC12 cells transfected with L61Cdc42 cDNA proliferated, whereas wild type Cdc42 and N17Cdc42 cDNA-transfected PC12 cells did not. When the cells were stimulated with NGF, ERK1/2 was transiently activated in L61Cdc42-transfected PC12 cells, whereas the activation of ERK1/2 was sustained in other cell lines. NGF repressed the induction of p21WAF1, cyclin-dependent kinase (CDK) inhibitor, in L61Cdc42 transfected PC12 cells, whereas NGF induced the expression of p21WAF1 in other PC12 cell lines.
Authors:
Ji-Yeon Seo; Jun-Sub Kim; Jeon-Ho Ghang; Tae-Cheon Kang; Jun-Gyo Suh; Eung-Gook Kim; Jong-Il Kim; Jaebong Kim; Jae-Yong Lee; Jae-Bong Park
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  14     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-25     Completed Date:  2003-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  1277-81     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, College of Medicine, Hallym University, Chunchon, Ockchon-Dong 1, Kangwon-Do, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects,  physiology
Humans
Nerve Growth Factor / pharmacology*
Neurites / drug effects*,  metabolism
PC12 Cells
Rats
cdc42 GTP-Binding Protein / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
9061-61-4/Nerve Growth Factor; EC 3.6.5.2/cdc42 GTP-Binding Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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