| Nerve growth factor induces the expression of functional FSH receptors in newly formed follicles of the rat ovary. | |
| | |
MedLine Citation:
|
PMID: 11897707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The neurotrophin nerve growth factor (NGF) and its two membrane-anchored receptors are expressed in the developing ovary before the organization of the first primordial follicles. In the absence of NGF, the growth of primordial follicles is retarded, indicating that NGF contributes to facilitating early follicular development. The present experiments were undertaken to determine whether NGF can also be involved in the differentiation process by which ovarian follicles become responsive to gonadotropins. Treatment of 2-d-old rat ovaries in organ culture with NGF increased FSH receptor (FSHR) mRNA within 8 h of exposure. This effect was cAMP-independent but additive to the cAMP-mediated increase in FSHR gene expression induced by either forskolin or vasoactive intestinal peptide, a neurotransmitter previously shown to induce FSHR formation in neonatal rat ovaries. After NGF treatment, the ovary acquired the capacity of responding to FSH with cAMP formation and preantral follicular growth, indicating that exposure to the neurotrophin resulted in the formation of biologically active FSHRs. Quantitative measurement of FSHR mRNA demonstrated that the content of FSHR mRNA is reduced in the ovaries of mice carrying a null mutation of the NGF gene. These results indicate that one of the functions of NGF in the developing ovary is to facilitate the differentiation process by which early growing follicles become gonadotropin-dependent during postnatal life, and that it does so by increasing the synthesis of FSHRs. |
| | |
Authors:
|
Carmen Romero; Alfonso Paredes; Gregory A Dissen; Sergio R Ojeda |
Related Documents
:
|
16039777 - Ndrg2 promotes neurite outgrowth of ngf-differentiated pc12 cells. 19387577 - Effect of korean red ginseng extract in a steroid-induced polycystic ovary murine model. 14522957 - How nerve growth factor drives physiological and inflammatory expressions of acid-sensi... 19458217 - The role of neurotrophins in the seasonal-like growth of the avian song control system. 7541537 - The choice of alternative 5' splice sites in influenza virus m1 mrna is regulated by th... 12387847 - Two casein kinase 1 isoforms are differentially expressed in trypanosoma cruzi. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Endocrinology Volume: 143 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2002 Apr |
Date Detail:
|
Created Date: 2002-03-18 Completed Date: 2002-04-29 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
|
Languages: eng Pagination: 1485-94 Citation Subset: AIM; IM |
Affiliation:
|
Division of Neuroscience, Oregon Regional Primate Research Center/Oregon Health Science University, Beaverton, Oregon 97006, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn / physiology Cyclic AMP / metabolism Female Gene Expression Regulation, Developmental / drug effects Nerve Growth Factor / pharmacology* Organ Culture Techniques Ovarian Follicle / drug effects, metabolism* Ovary / anatomy & histology, drug effects, metabolism* Pregnancy RNA, Messenger / biosynthesis, genetics Radioimmunoassay Rats Receptors, FSH / biosynthesis*, genetics Reverse Transcriptase Polymerase Chain Reaction Stimulation, Chemical Vasoactive Intestinal Peptide / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
HD-24870/HD/NICHD NIH HHS; RR-00163/RR/NCRR NIH HHS; TW HD-00668/TW/FIC NIH HHS; U54-HD-18185/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger; 0/Receptors, FSH; 37221-79-7/Vasoactive Intestinal Peptide; 60-92-4/Cyclic AMP; 9061-61-4/Nerve Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Localization and regulation of a functional GHRH receptor in the rat renal medulla.
Next Document: Caspase-3 is a pivotal mediator of apoptosis during regression of the ovarian corpus luteum.