Document Detail


Nerve growth factor induces the expression of functional FSH receptors in newly formed follicles of the rat ovary.
MedLine Citation:
PMID:  11897707     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The neurotrophin nerve growth factor (NGF) and its two membrane-anchored receptors are expressed in the developing ovary before the organization of the first primordial follicles. In the absence of NGF, the growth of primordial follicles is retarded, indicating that NGF contributes to facilitating early follicular development. The present experiments were undertaken to determine whether NGF can also be involved in the differentiation process by which ovarian follicles become responsive to gonadotropins. Treatment of 2-d-old rat ovaries in organ culture with NGF increased FSH receptor (FSHR) mRNA within 8 h of exposure. This effect was cAMP-independent but additive to the cAMP-mediated increase in FSHR gene expression induced by either forskolin or vasoactive intestinal peptide, a neurotransmitter previously shown to induce FSHR formation in neonatal rat ovaries. After NGF treatment, the ovary acquired the capacity of responding to FSH with cAMP formation and preantral follicular growth, indicating that exposure to the neurotrophin resulted in the formation of biologically active FSHRs. Quantitative measurement of FSHR mRNA demonstrated that the content of FSHR mRNA is reduced in the ovaries of mice carrying a null mutation of the NGF gene. These results indicate that one of the functions of NGF in the developing ovary is to facilitate the differentiation process by which early growing follicles become gonadotropin-dependent during postnatal life, and that it does so by increasing the synthesis of FSHRs.
Authors:
Carmen Romero; Alfonso Paredes; Gregory A Dissen; Sergio R Ojeda
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  143     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-03-18     Completed Date:  2002-04-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1485-94     Citation Subset:  AIM; IM    
Affiliation:
Division of Neuroscience, Oregon Regional Primate Research Center/Oregon Health Science University, Beaverton, Oregon 97006, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / physiology
Cyclic AMP / metabolism
Female
Gene Expression Regulation, Developmental / drug effects
Nerve Growth Factor / pharmacology*
Organ Culture Techniques
Ovarian Follicle / drug effects,  metabolism*
Ovary / anatomy & histology,  drug effects,  metabolism*
Pregnancy
RNA, Messenger / biosynthesis,  genetics
Radioimmunoassay
Rats
Receptors, FSH / biosynthesis*,  genetics
Reverse Transcriptase Polymerase Chain Reaction
Stimulation, Chemical
Vasoactive Intestinal Peptide / pharmacology
Grant Support
ID/Acronym/Agency:
HD-24870/HD/NICHD NIH HHS; RR-00163/RR/NCRR NIH HHS; TW HD-00668/TW/FIC NIH HHS; U54-HD-18185/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, FSH; 37221-79-7/Vasoactive Intestinal Peptide; 60-92-4/Cyclic AMP; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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