Document Detail

Nerve growth factor and glucocorticoids regulate phenotypic expression in cultured chromaffin cells from adult rhesus monkeys.
MedLine Citation:
PMID:  4065219     Owner:  NLM     Status:  MEDLINE    
Adrenal chromaffin cells and sympathetic neurons are both derivatives of the neural crest. Despite their morphological and functional differences, chromaffin cells retain some developmental plasticity and if treated with Nerve Growth Factor (NGF), can express certain characteristics of sympathetic neurons. However, there is some age and species variability in the response of chromaffin cells to NGF: in general chromaffin cells from adult animals are not considered to be dependent on NGF for survival, and chromaffin cells from adults of several species fail to respond to NGF in vitro by growing neurites. This is in contrast to the dramatic effects of NGF on chromaffin cells from perinatal rats. We have examined the requirements of chromaffin cells from adult rhesus monkeys to survive, to proliferate, and to express a neuronal morphology in vitro. NGF greatly enhances the proportion of rhesus chromaffin cells that form neurites and the length of the neurites that are formed, but the conversion to a neuronal phenotype is more limited than in chromaffin cells cultured from young rats. NGF also enhances rhesus chromaffin cell survival, but fails to stimulate their proliferation, in contrast to its effect on perinatal rat cells [18]. Glucocorticoid hormones (GCs) specifically antagonize the effects of NGF on neuritic outgrowth while promoting chromaffin cell survival. Thus adrenal chromaffin cells from rhesus monkeys retain a degree of developmental plasticity even in the adult animal.
L E Lillien; P Claude
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  161     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-01-06     Completed Date:  1986-01-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  255-68     Citation Subset:  IM    
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MeSH Terms
Adrenal Medulla / cytology
Age Factors
Catecholamines / analysis
Cell Division
Cell Survival
Cells, Cultured
Chromaffin System / analysis,  drug effects*,  ultrastructure
Dexamethasone / pharmacology*
Hydrocortisone / pharmacology*
Macaca mulatta
Nerve Growth Factors / antagonists & inhibitors,  pharmacology*
Neuronal Plasticity / drug effects
Grant Support
Reg. No./Substance:
0/Catecholamines; 0/Nerve Growth Factors; 50-02-2/Dexamethasone; 50-23-7/Hydrocortisone

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