| Nerve growth cones isolated from fetal rat brain: subcellular fractionation and characterization. | |
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MedLine Citation:
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PMID: 6652678 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The biochemical and functional characterization of the nerve growth cone is of major interest for studies on mechanisms involved in nervous system development. We describe the isolation from fetal brain of membrane-bound fragments of nerve growth cones by density gradient fractionation. These so-called growth cone particles are highly uniform and identifiable on the basis of their organelle complement. Furthermore, they co-purify in mixing experiments with fragments of radiolabeled and light microscopically identified nerve growth cones from primary cultures. The possibility of isolating growth cone fragments in quantity renders feasible the analysis of molecular mechanisms involved in growth cone function. |
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Authors:
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K H Pfenninger; L Ellis; M P Johnson; L B Friedman; S Somlo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell Volume: 35 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 1983 Dec |
Date Detail:
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Created Date: 1984-02-14 Completed Date: 1984-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 573-84 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / embryology*, ultrastructure Cell Fractionation / methods Centrifugation, Density Gradient / methods Female Fetus Microscopy, Electron Nerve Tissue Proteins / analysis Phospholipids / analysis Pregnancy Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Nerve Tissue Proteins; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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