Document Detail

Nerve growth cones isolated from fetal rat brain: subcellular fractionation and characterization.
MedLine Citation:
PMID:  6652678     Owner:  NLM     Status:  MEDLINE    
The biochemical and functional characterization of the nerve growth cone is of major interest for studies on mechanisms involved in nervous system development. We describe the isolation from fetal brain of membrane-bound fragments of nerve growth cones by density gradient fractionation. These so-called growth cone particles are highly uniform and identifiable on the basis of their organelle complement. Furthermore, they co-purify in mixing experiments with fragments of radiolabeled and light microscopically identified nerve growth cones from primary cultures. The possibility of isolating growth cone fragments in quantity renders feasible the analysis of molecular mechanisms involved in growth cone function.
K H Pfenninger; L Ellis; M P Johnson; L B Friedman; S Somlo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  35     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  573-84     Citation Subset:  IM    
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MeSH Terms
Brain / embryology*,  ultrastructure
Cell Fractionation / methods
Centrifugation, Density Gradient / methods
Microscopy, Electron
Nerve Tissue Proteins / analysis
Phospholipids / analysis
Rats, Inbred Strains
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Phospholipids

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