Document Detail

Nerve fibroblast impact on Schwann cell behavior.
MedLine Citation:
PMID:  19246119     Owner:  NLM     Status:  MEDLINE    
In order to reveal non-neuronal cell interactions after peripheral nerve lesions, we began to analyze the impact of sciatic nerve fibroblasts on Schwann cells in vitro. Both cell types are considered to have opposite effects on axonal regeneration. Few data are available on how repulsive nerve fibroblasts affect neuritotrophic Schwann cells and thus might indirectly influence axonal regrowth. Using different culture systems in conjunction with time-lapse video recording, metabolic labeling, pharmacological intervention, RNAi knockdown, Western blotting and RT-PCR analysis, we found that nerve fibroblasts differentially modify the various responses of Schwann cells. In the presence of collagen type IV and heparan sulfate proteoglycan but not of laminin, diffusible fibroblast factors slow down Schwann cell proliferation. In contrast, fibroblast factors increase the migratory activity of Schwann cells without being chemoattractive. One pro-migratory fibroblast factor turned out to be neuregulin. The pro-migratory activity of nerve fibroblasts and of recombinant neuregulin-1beta1 can be counteracted by neuregulin-specific pharmacological intervention and by neuregulin RNA interference. We show for the first time that nerve fibroblasts play antagonistic and agonistic roles for Schwann cells in a context-dependent manner. The data shed light on cellular mechanisms and have implications for some neuro-tissue engineering strategies.
Lars Dreesmann; Ursula Mittnacht; Martin Lietz; Burkhard Schlosshauer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-25
Journal Detail:
Title:  European journal of cell biology     Volume:  88     ISSN:  1618-1298     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-03-27     Completed Date:  2009-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  285-300     Citation Subset:  IM    
NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen, Reutlingen, Germany.
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MeSH Terms
Axons / physiology
Cell Communication / drug effects,  physiology*
Cell Differentiation / drug effects,  physiology
Cell Growth Processes / physiology
Cell Movement / drug effects,  physiology
Cells, Cultured
Extracellular Matrix / metabolism
Fibroblasts / cytology*,  drug effects,  metabolism
Gene Silencing
Nerve Regeneration
Neuregulin-1 / biosynthesis,  genetics,  metabolism,  pharmacology
RNA, Small Interfering
Rats, Inbred Lew
Schwann Cells / cytology*,  drug effects,  metabolism
Sciatic Nerve / cytology*
Signal Transduction
Reg. No./Substance:
0/Neuregulin-1; 0/RNA, Small Interfering

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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