Document Detail


Nerve growth factor promotes cardiac repair following myocardial infarction.
MedLine Citation:
PMID:  20360245     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: Nerve growth factor (NGF) promotes angiogenesis and cardiomyocyte survival, which are both desirable for postinfarction myocardial healing. Nonetheless, the NGF potential for cardiac repair has never been investigated.
OBJECTIVE: To define expression and localization of NGF and its high-affinity receptor TrkA (tropomyosin-related receptor A) in the human infarcted heart and to investigate the cardiac roles of both endogenous and engineered NGF using a mouse model of myocardial infarction (MI).
METHODS AND RESULTS: Immunostaining for NGF and TrkA was performed on heart samples from humans deceased of MI or unrelated pathologies. To study the post-MI functions of endogenous NGF, a NGF-neutralizing antibody (Ab-NGF) or nonimmune IgG (control) was given to MI mice. To investigate the NGF therapeutic potential, human NGF gene or control (empty vector) was delivered to the murine periinfarct myocardium. Results indicate that NGF is present in the infarcted human heart. Both cardiomyocytes and endothelial cells (ECs) possess TrkA, which suggests NGF cardiovascular actions in humans. In MI mice, Ab-NGF abrogated native reparative angiogenesis, increased EC and cardiomyocyte apoptosis and worsened cardiac function. Conversely, NGF gene transfer ameliorated EC and cardiomyocyte survival, promoted neovascularization and improved myocardial blood flow and cardiac function. The prosurvival/proangiogenic Akt/Foxo pathway mediated the therapeutic benefits of NGF transfer. Moreover, NGF overexpression increased stem cell factor (the c-kit receptor ligand) expression, which translated in higher myocardial abundance of c-kit(pos) progenitor cells in NGF-engineered hearts.
CONCLUSIONS: NGF elicits pleiotropic beneficial actions in the post-MI heart. NGF should be considered as a candidate for therapeutic cardiac regeneration.
Authors:
Marco Meloni; Andrea Caporali; Gallia Graiani; Costanza Lagrasta; Rajesh Katare; Sophie Van Linthout; Frank Spillmann; Ilaria Campesi; Paolo Madeddu; Federico Quaini; Costanza Emanueli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-01
Journal Detail:
Title:  Circulation research     Volume:  106     ISSN:  1524-4571     ISO Abbreviation:  Circ. Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-16     Completed Date:  2010-04-30     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1275-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Animals
Apoptosis
Autopsy
Case-Control Studies
Cells, Cultured
Coronary Circulation
Disease Models, Animal
Endothelial Cells / metabolism*,  pathology
Female
Forkhead Transcription Factors / metabolism
Genetic Therapy*
Humans
Male
Mice
Middle Aged
Myocardial Infarction / genetics,  metabolism,  pathology,  physiopathology,  therapy*
Myocardium / metabolism*,  pathology
Neovascularization, Physiologic
Nerve Growth Factor / biosynthesis*,  genetics
Proto-Oncogene Proteins c-akt / metabolism
Proto-Oncogene Proteins c-kit / metabolism
RNA, Messenger / biosynthesis
Rats
Rats, Wistar
Receptor, trkA / metabolism
Recombinant Proteins / biosynthesis
Regeneration* / genetics
Signal Transduction
Stem Cell Factor / metabolism
Stem Cells / metabolism,  pathology
Time Factors
Transfection
Ventricular Function, Left
Grant Support
ID/Acronym/Agency:
BS/05/001//British Heart Foundation; BS/05/001/18360//British Heart Foundation; FS/10/001/27959//British Heart Foundation; PG/06/146/21946//British Heart Foundation; PG/06/146/21946//British Heart Foundation
Chemical
Reg. No./Substance:
0/Forkhead Transcription Factors; 0/FoxO3 protein, mouse; 0/NGF protein, human; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Stem Cell Factor; 9061-61-4/Nerve Growth Factor; EC 2.7.10.1/Proto-Oncogene Proteins c-kit; EC 2.7.10.1/Receptor, trkA; EC 2.7.11.1/Proto-Oncogene Proteins c-akt
Comments/Corrections

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