| Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor. | |
| | |
MedLine Citation:
|
PMID: 10091924 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Biodegradable microspheres were prepared through glutaraldehyde cross-linking of gelatin without using any surfactants as a carrier matrix of basic fibroblast growth factor (bFGF). In the in vitro system, bFGF was sorbed to microspheres of acidic gelatin with an isoelectric point (IEP) of 5.0, but not to those of basic gelatin with an IEP of 9.0. The rate of bFGF sorption to the acidic gelatin microsphere in phosphate-buffered saline solution (pH 7.4) was smaller than that in water. Following incorporation of bFGF into the microspheres at 4 degrees C for 12 h, bFGF release from the bFGF-incorporating microspheres was studied. Approximately 30% of incorporated bFGF was released from the acidic gelatin microsphere within the initial 3 h, followed by no substantial release, whereas the basic gelatin microsphere released almost completely the incorporated bFGF within 1 day. It is likely that when basic bFGF molecules were immobilized to the acidic gelatin constituting microspheres through polyion complexation, they were not readily released under the in vitro nondegradation condition of gelatin. Incorporation of anionic carboxylmethyl cellulose (CMC) into the acidic gelatin microspheres reduced the amount of bFGF desorbed initially. This indicates that the initial burst is ascribed to free bFGF which is not ionically interacted with the acidic gelatin. CMC will function as a bFGF sorbent to suppress the initial leakage from the microspheres. When injected subcutaneously into the mouse back, bFGF-incorporating acidic gelatin microspheres were degraded over time and induced neovascularization around the injection site, in marked contrast to bFGF in the solution form. CMC incorporation slowed down the biodegradation and vascularization effect of bFGF-incorporating gelatin microspheres. It was concluded that the gelatin microsphere was a promising carrier matrix of bFGF to enhance the vascularization effect. |
| | |
Authors:
|
Y Tabata; S Hijikata; M Muniruzzaman; Y Ikada |
Related Documents
:
|
10605094 - In-situ etching observation of human teeth in acid agent by atomic force microscopy. 12732514 - Cloning and sequencing of a poly(dl-lactic acid) depolymerase gene from paenibacillus a... 12001244 - Dentin proteoglycans: an immunocytochemical feisem study. 2092824 - The effect of the l-azetidine-2-carboxylic acid residue on protein conformation. ii. ho... 3468524 - Effect of ovariectomy in humans on serum 6-keto-pgf1 alpha and txb2 concentrations and ... 8035644 - Synthetic human adrenomedullin and adrenomedullin 15-52 have potent short-lived vasodil... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of biomaterials science. Polymer edition Volume: 10 ISSN: 0920-5063 ISO Abbreviation: J Biomater Sci Polym Ed Publication Date: 1999 |
Date Detail:
|
Created Date: 1999-06-01 Completed Date: 1999-06-01 Revised Date: 2008-02-20 |
Medline Journal Info:
|
Nlm Unique ID: 9007393 Medline TA: J Biomater Sci Polym Ed Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 79-94 Citation Subset: IM |
Affiliation:
|
Research Center for Biomedical Engineering, Kyoto University, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carboxymethylcellulose / chemistry Chromatography, High Pressure Liquid Delayed-Action Preparations* Drug Delivery Systems / methods* Excipients / chemistry, pharmacology Fibroblast Growth Factor 2 / administration & dosage, pharmacology* Gelatin / chemistry, pharmacology Hemoglobins / analysis Hydrogels Injections, Subcutaneous Isoelectric Point Mice Microspheres Neovascularization, Physiologic / drug effects* Recombinant Proteins / administration & dosage, pharmacology Skin / blood supply |
| Chemical | |
Reg. No./Substance:
|
0/Delayed-Action Preparations; 0/Excipients; 0/Hemoglobins; 0/Hydrogels; 0/Recombinant Proteins; 103107-01-3/Fibroblast Growth Factor 2; 9000-70-8/Gelatin; 9004-32-4/Carboxymethylcellulose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro evaluation of cytotoxicity of a naturally occurring cross-linking reagent for biological ti...
Next Document: Cytomimetic biomaterials. 3. Preparation and transport studies of an alginate/amphiphilic copolymer/...