| Neospora caninum-infected cattle develop parasite-specific CD4+ cytotoxic T lymphocytes. | |
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MedLine Citation:
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PMID: 12761108 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cattle infected with Neospora caninum readily experience transplacental parasite transmission, presumably after maternal parasitemia, leading to abortion or birth of congenitally infected calves. Cytotoxic T lymphocytes (CTL) are important mediators of protective immunity against Toxoplasma gondii, an intracellular apicomplexan protozoan closely related to N. caninum. In this study, N. caninum-specific CTL expanded from peripheral blood mononuclear cells of two major histocompatibility complex-mismatched, experimentally infected cattle were identified by using a (51)Cr release cytotoxicity assay. Enrichment and blocking of CD4(+)- and CD8(+)-T-lymphocyte effector subsets indicated that CD4(+) CTL killed N. caninum-infected, autologous target cells and that killing was mediated through a perforin/granzyme pathway. Detection and characterization of CTL responses to N. caninum in the natural, outbred, bovine host will facilitate identification of immunogens and design of immunization strategies to induce parasite-specific CTL against transplacental N. caninum transmission in cattle. |
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Authors:
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Lauren M Staska; Travis C McGuire; Christopher J Davies; Harris A Lewin; Timothy V Baszler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 71 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-05-22 Completed Date: 2003-06-19 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 3272-9 Citation Subset: IM |
Affiliation:
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Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / therapeutic use Antigens, CD4 / analysis* Cattle Coccidiosis / immunology* Female Granzymes Membrane Glycoproteins / physiology Neospora / immunology* Perforin Pore Forming Cytotoxic Proteins Serine Endopeptidases / physiology T-Lymphocytes, Cytotoxic / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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1K08 AI49182-01/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD4; 0/Membrane Glycoproteins; 0/Pore Forming Cytotoxic Proteins; 126465-35-8/Perforin; EC 3.4.21.-/Granzymes; EC 3.4.21.-/Serine Endopeptidases |
| Comments/Corrections | |
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