Document Detail

Neospora caninum antigens defined by antigen-dependent bovine CD4+ T cells.
MedLine Citation:
PMID:  16108548     Owner:  NLM     Status:  MEDLINE    
Neosporosis is an important cause of pregnancy loss in cattle worldwide. The objective of the present study was to identify Neospora caninum antigens as vaccine candidates using antigen-specific, short-term CD4+ T cells established from N. caninum-immunized and -challenged cows. Whole N. caninum tachyzoite lysate was separated into 6 fractions by DEAE anion-exchange chromatography using high-pressure liquid chromatography (HPLC). The CD4+ T-cell proliferation assay results indicated that antigenic activity was associated with proteins from HPLC fractions 4-6, with fraction 5 exhibiting the highest antigenic activity. Also, SDS-PAGE analysis revealed a 16-kDa protein in fractions 4-6 that was recognized by anti-N. caninum antibodies. This 16-kDa protein was absent in other fractions, and it may be a target of a T-cell response in cattle. Further identification of immunogenic proteins of N. caninum may facilitate development of subunit vaccines against neosporosis.
Wenbin Tuo; Raymond H Fetterer; William C Davis; Mark C Jenkins; J P Dubey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of parasitology     Volume:  91     ISSN:  0022-3395     ISO Abbreviation:  J. Parasitol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-08-19     Completed Date:  2005-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7803124     Medline TA:  J Parasitol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  564-8     Citation Subset:  IM    
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, USA.
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MeSH Terms
Antigens, Protozoan / analysis,  immunology*
Blotting, Western / veterinary
CD4-Positive T-Lymphocytes / immunology*
Cattle Diseases / immunology,  prevention & control
Chromatography, DEAE-Cellulose / veterinary
Chromatography, High Pressure Liquid / veterinary
Coccidiosis / immunology,  prevention & control,  veterinary
Electrophoresis, Polyacrylamide Gel / veterinary
Lymphocyte Activation
Neospora / immunology*
Protozoan Proteins / analysis,  immunology
Protozoan Vaccines / immunology
Silver Staining / veterinary
Vaccines, Subunit / immunology
Reg. No./Substance:
0/Antigens, Protozoan; 0/Protozoan Proteins; 0/Protozoan Vaccines; 0/Vaccines, Subunit

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