| Neospora caninum antigens defined by antigen-dependent bovine CD4+ T cells. | |
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MedLine Citation:
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PMID: 16108548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neosporosis is an important cause of pregnancy loss in cattle worldwide. The objective of the present study was to identify Neospora caninum antigens as vaccine candidates using antigen-specific, short-term CD4+ T cells established from N. caninum-immunized and -challenged cows. Whole N. caninum tachyzoite lysate was separated into 6 fractions by DEAE anion-exchange chromatography using high-pressure liquid chromatography (HPLC). The CD4+ T-cell proliferation assay results indicated that antigenic activity was associated with proteins from HPLC fractions 4-6, with fraction 5 exhibiting the highest antigenic activity. Also, SDS-PAGE analysis revealed a 16-kDa protein in fractions 4-6 that was recognized by anti-N. caninum antibodies. This 16-kDa protein was absent in other fractions, and it may be a target of a T-cell response in cattle. Further identification of immunogenic proteins of N. caninum may facilitate development of subunit vaccines against neosporosis. |
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Authors:
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Wenbin Tuo; Raymond H Fetterer; William C Davis; Mark C Jenkins; J P Dubey |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The Journal of parasitology Volume: 91 ISSN: 0022-3395 ISO Abbreviation: J. Parasitol. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-08-19 Completed Date: 2005-08-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7803124 Medline TA: J Parasitol Country: United States |
Other Details:
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Languages: eng Pagination: 564-8 Citation Subset: IM |
Affiliation:
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Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, USA. wtuo@anri.barc.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Protozoan / analysis, immunology* Blotting, Western / veterinary CD4-Positive T-Lymphocytes / immunology* Cattle Cattle Diseases / immunology, prevention & control Chromatography, DEAE-Cellulose / veterinary Chromatography, High Pressure Liquid / veterinary Coccidiosis / immunology, prevention & control, veterinary Electrophoresis, Polyacrylamide Gel / veterinary Female Lymphocyte Activation Neospora / immunology* Protozoan Proteins / analysis, immunology Protozoan Vaccines / immunology Rabbits Silver Staining / veterinary Vaccines, Subunit / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Protozoan; 0/Protozoan Proteins; 0/Protozoan Vaccines; 0/Vaccines, Subunit |
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