Document Detail

Neoplastic transformation of human diploid fibroblasts after long-term serum starvation.
MedLine Citation:
PMID:  16488534     Owner:  NLM     Status:  MEDLINE    
Serum starvation for several days has been considered as a positive effect on the efficiency of nuclear transfer using donor cells. The effects of longer period serum starvation are not clear while similar starvation might occur in vitro maintained cells (i.e. tissue engineering products) and in vivo such as ischemia of human tissues or organs. We found human dermis fibroblasts were transformed for about 70 days caused by serum starvation (0.5% serum). The transformed cells became round and had more than one nucleolus. In 0.5% serum medium they kept almost constant growth rate as the normal fibroblasts in 10% serum medium. Abnormal karyotype including aneuploidy and structural aberrations was observed. The transformed cells had high telomerase activities, in contrast, normal fibroblasts had no detectable telomerase activities. C-myc was up-regulated while cdk2, cyclin A, p21 were down in transformed cells. Cell transplantation into SCID nude mice confirmed that the cells had the capacity of forming solid tumors. The results indicated that long-term serum starvation could lead to cell chromosomal instability and transformation.
Jingyu Zhang; Xia Wang; Yanhong Zhao; Bing Chen; Guangli Suo; Jianwu Dai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-20
Journal Detail:
Title:  Cancer letters     Volume:  243     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-09     Completed Date:  2006-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  101-8     Citation Subset:  IM    
Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No. 3 Nanyitiao, Zhongguancun, Beijing 100080, China.
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MeSH Terms
Cell Cycle Proteins / genetics
Cell Proliferation / drug effects
Cell Size / drug effects
Cell Transformation, Neoplastic / drug effects,  genetics,  pathology*
Cells, Cultured
Culture Media, Serum-Free / pharmacology
Fibroblasts / cytology*,  metabolism,  transplantation
Gene Expression / drug effects
Mice, Nude
Mice, SCID
Neoplasms, Experimental / genetics,  metabolism,  pathology
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Telomerase / metabolism
Time Factors
Transplantation, Heterologous
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Culture Media, Serum-Free; 0/RNA, Messenger; EC

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