Document Detail


Neoplastic transformation and cytogenetic changes after Gamma irradiation of human epithelial cells expressing telomerase.
MedLine Citation:
PMID:  11121238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neoplastic transformation of human epithelial cells by radiation has previously been investigated using cell lines immortalized with viral vectors. There are disadvantages to this approach, and we report here the results of studies using a human retinal pigment epithelial cell line (340RPE-T53) immortalized by treatment with telomerase. After exposure of the cells to fractionated doses of gamma radiation, there was a marked increase in anchorage-independent growth of the surviving cells. The cloned cell lines derived from these anchorage-independent cultures exhibited an increased growth rate in vitro and were serum-independent compared with the parent cell line. The parent cell line maintained a stable diploid karyotype. The cell lines cloned after irradiation with the lower doses (10 x 2 Gy) were hypodiploid with loss of chromosome 13 and a high level amplification of 10p11.2 associated with a deletion of the remaining short arm segment of chromosome 10 distal to 10p11.2. In contrast, the cell lines cloned after irradiation with the higher doses (15 x 2 Gy) were near-tetraploid with derivative chromosomes present characterized by SKY analysis. Thus this human epithelial cell line immortalized with telomerase provides an improved model to investigate mechanisms of radiation carcinogenesis.
Authors:
A Riches; C Peddie; S Rendell; P Bryant; H Zitzelsberger; J Bruch; J Smida; L Hieber; M Bauchinger
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation research     Volume:  155     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  222-229     Citation Subset:  IM; S    
Affiliation:
School of Biology, Medical Science & Human Biology, University of St. Andrews, St. Andrews KY16 9TS, Scotland, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion / physiology
Cell Division / radiation effects
Cell Line
Cell Transformation, Neoplastic / genetics*,  radiation effects*
Chromosome Deletion
Gamma Rays
Genotype
Humans
Pigment Epithelium of Eye / cytology,  enzymology,  radiation effects*
Telomerase / biosynthesis*
Chemical
Reg. No./Substance:
EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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