Document Detail

Neoplastic transformation by Notch requires nuclear localization.
MedLine Citation:
PMID:  10805736     Owner:  NLM     Status:  MEDLINE    
Notch proteins are plasma membrane-spanning receptors that mediate important cell fate decisions such as differentiation, proliferation, and apoptosis. The mechanism of Notch signaling remains poorly understood. However, it is clear that the Notch signaling pathway mediates its effects through intercellular contact between neighboring cells. The prevailing model for Notch signaling suggests that ligand, presented on a neighboring cell, triggers proteolytic processing of Notch. Following proteolysis, it is thought that the intracellular portion of Notch (N(ic)) translocates to the nucleus, where it is involved in regulating gene expression. There is considerable debate concerning where in the cell Notch functions and what proteins serve as effectors of the Notch signal. Several Notch genes have clearly been shown to be proto-oncogenes in mammalian cells. Activation of Notch proto-oncogenes has been associated with tumorigenesis in several human and other mammalian cancers. Transforming alleles of Notch direct the expression of truncated proteins that primarily consist of N(ic) and are not tethered to the plasma membrane. However, the mechanism by which Notch oncoproteins (generically termed here as N(ic)) induce neoplastic transformation is not known. Previously we demonstrated that N1(ic) and N2(ic) could transform E1A immortalized baby rat kidney cells (RKE) in vitro. We now report direct evidence that N1(ic) must accumulate in the nucleus to induce transformation of RKE cells. In addition, we define the minimal domain of N1(ic) required to induce transformation and present evidence that transformation of RKE cells by N1(ic) is likely to be through a CBF1-independent pathway.
S Jeffries; A J Capobianco
Related Documents :
12015986 - Prickle mediates feedback amplification to generate asymmetric planar cell polarity sig...
2911386 - A mutation that changes cell movement and cell fate in the zebrafish embryo.
20560956 - Notch signalling in primary cutaneous cd30+ lymphoproliferative disorders: a new therap...
8755476 - Control of daughter cell fates during asymmetric division: interaction of numb and notch.
24223636 - Human amniotic epithelial cell feeder layers maintain ips cell pluripotency by inhibiti...
15049366 - Production of l-phenylacetyl carbinol by immobilized cells of saccharomyces cerevisiae.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  20     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3928-41     Citation Subset:  IM    
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0524, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Binding Sites
Cell Division
Cell Line
Cell Line, Transformed
Cell Nucleus / metabolism
Cell Transformation, Neoplastic*
Culture Media
DNA-Binding Proteins / genetics,  metabolism
Gene Expression
Hela Cells
Immunoglobulin J Recombination Signal Sequence-Binding Protein
Membrane Proteins / genetics*,  metabolism
Nuclear Proteins*
Receptor, Notch1
Receptors, Cell Surface*
Transcription Factors*
Reg. No./Substance:
0/Culture Media; 0/DNA-Binding Proteins; 0/Immunoglobulin J Recombination Signal Sequence-Binding Protein; 0/Membrane Proteins; 0/NOTCH1 protein, human; 0/Notch1 protein, rat; 0/Nuclear Proteins; 0/RBPJ protein, human; 0/Rbpsuh protein, rat; 0/Receptor, Notch1; 0/Receptors, Cell Surface; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Role of Saccharomyces cerevisiae ISA1 and ISA2 in iron homeostasis.
Next Document:  Mutational analysis of mammalian translation initiation factor 5 (eIF5): role of interaction between...