Document Detail

Neoplastic and non-neoplastic cell lines from a malignant peripheral nerve sheath tumour of the cervix of a rat.
MedLine Citation:
PMID:  17537454     Owner:  NLM     Status:  MEDLINE    
A homotransplantable tumour (LY) and cell lines (LY-PPB6 and LY-H12) were established from a spontaneous malignant peripheral nerve sheath tumour (PNST) of the uterine cervix of an F344 rat. Primary and LY tumours consisted of oval or spindle-shaped cells arranged in a flatfield or streaming fashion, and indistinct nuclear palisades were seen. Immunohistochemically, neoplastic cells reacted to vimentin, S-100 protein, neuron-specific enolase (NSE), myelin basic protein (MBP), and glial fibrillary acidic protein (GFAP) in varying degrees, indicating neurogenic derivation. LY-PPB6-induced tumours in syngeneic rats developed cellular whorling patterns reacting particularly strongly to S-100 protein, NSE, MBP and GFAP. Nerve growth factor (NGF) mRNA expression was shown in LY-PPB6 cells by the reverse transcription-polymerase chain reaction (RT-PCR). By contrast, LY-H12 had a normal chromosomal number of 42, and did not produce tumours when injected into syngeneic rats. LY-H12 cells reacted to vimentin and alpha-smooth muscle actin (alpha-SMA), and the alpha-SMA-positive cell number was increased dose-dependently by the addition of transforming growth factor (TGF)-beta1, indicating a myofibroblastic nature. LY-PPB6 cells were neoplastic with properties of PNS cells, whereas LY-H12 cells were non-neoplastic stromal cells showing myofibroblastic differentiation. As TGF-beta1 mRNA expression was shown in both LY-PPB6 and LY-H12 cells by the RT-PCR, the myofibroblastic phenotype of LY-H12 cells may be mediated by paracrine or autocrine signalling in tumour tissues. LY-PPB6 and LY-H12 may prove useful for studies on the pathobiological nature of neoplastic cells and interactions between neoplastic and stromal cells in PNSTs.
J Yamate; M Sakamori; M Kuwamura; T Kotani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-29
Journal Detail:
Title:  Journal of comparative pathology     Volume:  137     ISSN:  0021-9975     ISO Abbreviation:  J. Comp. Pathol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-16     Completed Date:  2007-09-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0102444     Medline TA:  J Comp Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  9-21     Citation Subset:  IM    
Laboratory of Veterinary Pathology, Life and Environmental Sciences, Osaka Prefecture University, Gakuencho 1-1, Sakai, Osaka 599-8531, Japan.
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MeSH Terms
Actins / genetics,  metabolism
Cell Line
Cell Line, Tumor
Disease Models, Animal
Dose-Response Relationship, Drug
Gene Expression Regulation, Neoplastic / drug effects
Nerve Growth Factor / genetics,  metabolism
Nerve Sheath Neoplasms / metabolism,  pathology*,  veterinary
RNA, Messenger / genetics,  metabolism
Rats, Inbred F344
Stromal Cells / metabolism,  pathology*
Transforming Growth Factor beta1 / pharmacology
Uterine Cervical Neoplasms / metabolism,  pathology*,  veterinary
Reg. No./Substance:
0/Actins; 0/RNA, Messenger; 0/Transforming Growth Factor beta1; 9061-61-4/Nerve Growth Factor

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