| Neonatal testicular gonocytes isolation and processing for immunocytochemical analysis. | |
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MedLine Citation:
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PMID: 22144233 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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In recent years, an increasing interest has emerged at understanding how spermatogonial stem cells (SSCs) arise from their precursor cells, the gonocytes. The identification of factors acting directly on gonocytes rather than on the surrounding somatic cells and the study of genes and signaling pathways intrinsic to gonocytes require their isolation from Sertoli and peritubular cells. The present article describes a simple method developed to isolate rat neonatal gonocytes, allowing for the study of their proliferation and differentiation to SSCs. We also present immunocytochemical methods that can be used to study protein expression changes and proliferation in gonocytes. |
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Authors:
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Gurpreet Manku; Monty Mazer; Martine Culty |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 825 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-12-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 17-29 Citation Subset: IM |
Affiliation:
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The Research Institute of the McGill University Health Centre and Departments of Medicine and Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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