Document Detail


Neonatal phosphate nutrition alters in vivo and in vitro satellite cell activity in pigs.
MedLine Citation:
PMID:  22822445     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO(4)) deficiency can alter satellite cell activity, however the role of neonatal PO(4) nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW) were pair-fed liquid diets that were either PO(4) adequate (0.9% total P), supra-adequate (1.2% total P) in PO(4) requirement or deficient (0.7% total P) in PO(4) content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured in vitro for 7 days to determine if PO(4) nutrition alters their ability to proceed through their myogenic lineage. Dietary PO(4) deficiency resulted in reduced (P < 0.05) sera PO(4) and parathyroid hormone (PTH) concentrations, while supra-adequate dietary PO(4) improved (P < 0.05) feed conversion efficiency as compared to the PO(4) adequate group. In vivo satellite cell proliferation was reduced (P < 0.05) among the PO(4) deficient pigs, and these cells had altered in vitro expression of markers of myogenic progression. Further work to better understand early nutritional programming of satellite cells and the potential benefits of emphasizing early PO(4) nutrition for future lean growth potential is warranted.
Authors:
Lindsey S Alexander; Brynn S Seabolt; Robert P Rhoads; Chad H Stahl
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Publication Detail:
Type:  Journal Article     Date:  2012-05-31
Journal Detail:
Title:  Nutrients     Volume:  4     ISSN:  2072-6643     ISO Abbreviation:  Nutrients     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-07-23     Completed Date:  2012-12-12     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101521595     Medline TA:  Nutrients     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  436-48     Citation Subset:  IM    
Affiliation:
Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC 27695, USA. lindsey.alexander1@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animal Feed
Animal Nutritional Physiological Phenomena
Animals
Animals, Newborn / growth & development
Body Weight / drug effects,  physiology*
Case-Control Studies
Cell Proliferation / drug effects
Energy Intake
Female
Male
Parathyroid Hormone / blood
Phosphates / blood,  deficiency*
Phosphorus, Dietary / administration & dosage*,  pharmacology
Satellite Cells, Skeletal Muscle / metabolism*
Swine
Chemical
Reg. No./Substance:
0/Parathyroid Hormone; 0/Phosphates; 0/Phosphorus, Dietary
Comments/Corrections

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