| Neonatal pancreatic cells redifferentiate into both neural and pancreatic lineages. | |
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MedLine Citation:
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PMID: 17109820 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies of islet neogenesis have suggested that the regeneration of islet cells can be achieved through redifferentiation of pre-existing islet cells. However, this hypothesis is largely unproven and fails to account for the diversity of observed islet neogenesis. Here we show that cultured neonatal pancreatic cells dedifferentiate into betaIII tubulin-expressing precursors, which then expand and redifferentiate into both neural and pancreatic lineage progenies. Redifferentiation happens not only in the islet cells, but also in the ductal cells that may represent what are called ductal origin "pancreatic stem cells". The in vitro redifferentiation of neonatal pancreatic cells recapitulates the embryonic development by sequential endocrine differentiation accompanied by the coexpression of neuronal marker betaIII tubulin with endocrine hormones until terminal differentiation. The neuronal differentiation of pancreatic cells, however, occurs prior to endocrine differentiation and gradually becomes dominant, thus implying a default neuronal lineage specification for cultured pancreatic cells. |
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Authors:
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Wenping Zhao; Tomonori Hirose; Momotaro Ishikawa; Yuji Oshima; Syu-Ichi Hirai; Shigeo Ohno; Hideki Taniguchi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-10 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 352 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-04 Completed Date: 2007-02-01 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 84-90 Citation Subset: IM |
Affiliation:
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Department of Regenerative Medicine, Graduate School of Medical Science, Yokohama City University, Yokohama 236-0004, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Biological Markers Cell Differentiation* Cell Lineage* Cell Separation Cells, Cultured Insulin / genetics Mice Mice, Inbred C57BL Neurons / cytology* Pancreas / cytology*, metabolism Promoter Regions, Genetic / genetics Stem Cells / cytology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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