Document Detail


Neonatal dexamethasone treatment increases susceptibility to experimental autoimmune disease in adult rats.
MedLine Citation:
PMID:  11067955     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Major concern has emerged about the possible long term adverse effects of glucocorticoid treatment, which is frequently used for the prevention of chronic lung disease in preterm infants. Here we show that neonatal glucocorticoid treatment of rats increases the severity (p< or = 0.01) and incidence (p< or =0.01) of the inflammatory autoimmune disease experimental autoimmune encephalomyelitis in adult life. In search of possible mechanisms responsible for the increased susceptibility to experimental autoimmune encephalomyelitis, we investigated the reactivity of the hypothalamo-pituitary-adrenal axis and of immune cells in adult rats after neonatal glucocorticoid treatment. We observed that neonatal glucocorticoid treatment reduces the corticosterone response after an LPS challenge in adult rats (p< or =0.001). Interestingly, LPS-stimulated macrophages of glucocorticoid-treated rats produce less TNF-alpha and IL-1beta in adult life than control rats (p<0.05). In addition, splenocytes obtained from adult rats express increased mRNA levels of the proinflammatory cytokines IFN-gamma (p<0.01) and TNF-beta (p<0.05) after neonatal glucocorticoid treatment. Apparently, neonatal glucocorticoid treatment has permanent programming effects on endocrine as well as immune functioning in adult life. In view of the frequent clinical application of glucocorticoids to preterm infants, our data demonstrate that neonatal glucocorticoid treatment may be a risk factor for the development of (auto)immune disease in man.
Authors:
J M Bakker; A Kavelaars; P J Kamphuis; P M Cobelens; H H van Vugt; F van Bel; C J Heijnen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  165     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-11-21     Completed Date:  2000-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5932-7     Citation Subset:  AIM; IM    
Affiliation:
Departments of. Pediatric Immunology and Neonatology, Wilhelmina Children's Hospital of the University Medical Center, and Rudolf Magnus Institute, Utrecht, The Netherlands. j.m.bakker-2@wkz.azu.nl
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MeSH Terms
Descriptor/Qualifier:
Aging / blood,  immunology*
Animals
Animals, Newborn / growth & development,  immunology*
Body Weight / drug effects
Cells, Cultured
Corticosterone / antagonists & inhibitors,  blood
Cytokines / biosynthesis,  genetics
Dexamethasone / administration & dosage*,  adverse effects*
Disease Susceptibility
Encephalomyelitis, Autoimmune, Experimental / blood,  epidemiology,  immunology*,  physiopathology
Female
Immunosuppressive Agents / administration & dosage,  adverse effects
Incidence
Injections, Intraperitoneal
Interleukin-1 / antagonists & inhibitors,  biosynthesis
Lipopolysaccharides / administration & dosage,  antagonists & inhibitors
Lymphocyte Activation / drug effects
Macrophages, Peritoneal / immunology,  metabolism
Male
RNA, Messenger / biosynthesis
Rats
Rats, Wistar
Severity of Illness Index
Spleen / cytology,  drug effects,  immunology
Tumor Necrosis Factor-alpha / antagonists & inhibitors,  biosynthesis
Chemical
Reg. No./Substance:
0/Cytokines; 0/Immunosuppressive Agents; 0/Interleukin-1; 0/Lipopolysaccharides; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; 50-02-2/Dexamethasone; 50-22-6/Corticosterone

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