| Neomycin improves cationic lipid-mediated transfection of DNA in human cells. | |
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MedLine Citation:
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PMID: 15950473 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Delivery of oligonucleotides has been a major impediment in the development of nucleic acid based drugs. In this report, we show that neomycin, an aminoglycoside antibiotic, when combined with a cationic lipid preparation such as DOTAP, enhances transfection efficiency of both reporter plasmids and oligonucleotides and results in a significant increase in transgene expression. The results described here open a new lead in ongoing efforts for oligonucleotide delivery. |
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Authors:
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Sara Napoli; Giuseppina M Carbone; Carlo V Catapano; Nick Shaw; Dev P Arya |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioorganic & medicinal chemistry letters Volume: 15 ISSN: 0960-894X ISO Abbreviation: Bioorg. Med. Chem. Lett. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-06-24 Completed Date: 2005-12-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9107377 Medline TA: Bioorg Med Chem Lett Country: England |
Other Details:
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Languages: eng Pagination: 3467-9 Citation Subset: IM |
Affiliation:
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Laboratory of Experimental Oncology, Oncology Institute of Southern Switzerland (IOSI) Via Vela 6, 6500 Bellinzona, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Sequence Cations / chemistry Cell Line, Tumor DNA / chemistry* Drug Delivery Systems Fatty Acids, Monounsaturated / chemistry Humans Lipids / chemistry* Male Molecular Sequence Data Molecular Structure Neomycin / chemistry* Prostatic Neoplasms / metabolism* Quaternary Ammonium Compounds / chemistry Transfection / methods* |
| Chemical | |
Reg. No./Substance:
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0/Cations; 0/Fatty Acids, Monounsaturated; 0/Lipids; 0/Quaternary Ammonium Compounds; 113669-21-9/1,2-dioleoyloxy-3-(trimethylammonium)propane; 1404-04-2/Neomycin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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