| Neointimal formation by circulating bone marrow cells. | |
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MedLine Citation:
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PMID: 11795265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The origin of smooth muscle cells involved in vascular healing was examined. Eighteen C57BL/6 (Ly 5.2) female mice underwent whole body irradiation followed by transfusion with 106 bone nucleated marrow cells from congenic (Ly 5.1) male donors. Successful repopulation by donor marrow was demonstrated after 4 weeks by flow cytometry with FITC-conjugated A20.1/Ly 5.1 monoclonal antibody. The iliac artery of six of the chimeric mice was scratch-injured by five passes of a probe, causing severe medial damage. After 4 weeks the arterial lumen was obliterated by a cell-rich neointima, with alpha-smooth muscle actin-containing cells present around the residual lumen. Approximately half of these cells were of male donor origin, as evidenced by in situ hybridization with a Y chromosome-specific probe. An organized arterial thrombus was formed in the remaining 12 chimeric mice by inserting an 8-0 silk suture into the left common carotid artery. Donor cells staining with alpha-smooth muscle actin were found in those arteries sustaining serious damage but not in arteries with minimal damage. Our results suggest that bone marrow-derived cells are recruited in vascular healing as a complementary source of smooth muscle-like cells when the media is severely damaged and few resident smooth muscle cells are available to effect repair. |
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Authors:
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J H Campbell; C L Han; G R Campbell |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Annals of the New York Academy of Sciences Volume: 947 ISSN: 0077-8923 ISO Abbreviation: Ann. N. Y. Acad. Sci. Publication Date: 2001 Dec |
Date Detail:
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Created Date: 2002-01-17 Completed Date: 2002-02-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7506858 Medline TA: Ann N Y Acad Sci Country: United States |
Other Details:
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Languages: eng Pagination: 18-24; discussion 24-5 Citation Subset: IM |
Affiliation:
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Centre for Research in Vascular Biology, University of Queensland, Brisbane, Australia. julie.campbell@mailbox.uq.edu.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arteriosclerosis
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pathology,
physiopathology Bone Marrow Cells / cytology*, pathology Cell Movement Humans Tunica Intima / cytology*, pathology Wounds and Injuries / pathology, physiopathology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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